Processing

Please wait...

Settings

Settings

Goto Application

1. WO2016029122 - USE OF JAGGED 1/FRIZZLED 4 AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS

Publication Number WO/2016/029122
Publication Date 25.02.2016
International Application No. PCT/US2015/046309
International Filing Date 21.08.2015
IPC
C12N 5/077 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
CPC
A61K 35/34
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
34Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
A61P 13/12
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
13Drugs for disorders of the urinary system
12of the kidneys
A61P 9/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
A61P 9/10
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
10for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
C12N 2501/415
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
40Regulators of development
415Wnt; Frizzeled
C12N 2501/42
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
40Regulators of development
42Notch; Delta; Jagged; Serrate
Applicants
  • PROCELLA THERAPEUTICS AB [SE]/[SE]
Inventors
  • CHIEN, Kenneth, R.
  • LIAN, Xiaojun
Agents
  • REMILLARD, Jane, E.
Priority Data
62/040,89222.08.2014US
62/194,01617.07.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) USE OF JAGGED 1/FRIZZLED 4 AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS
(FR) UTILISATION DE JAGGED 1/FRIZZLED 4 À TITRE DE MARQUEURS DE SURFACE CELLULAIRE POUR ISOLER DES CELLULES PROGÉNITRICES VENTRICULAIRES CARDIAQUES HUMAINES
Abstract
(EN)
The present invention provides Jagged 1 and Frizzled 4 as cell surface markers for isolating human cardiomyogenic ventricular progenitor cells, in particular progenitor cells that preferentially differentiate into cardiac ventricular muscle cells. Thus, the invention provides human ventricular progenitor (HVP) cells. The invention provides in vitro methods of the separation of Islet 1+ Jagged 1+ ventricular progenitor cells and/or Islet 1+/Frizzled 4+ ventricular progenitor cells and/or Islet 1+/Jagged 1+/Frizzled 4+ ventricular progenitor cells, and the large scale expansion and propagation thereof. Large clonal populations of isolated Jagged 1+ and/or Frizzled 4+ ventricular progenitor cells are also provided. Methods of in vivo use of Jagged 1+ and/or Frizzled 4+ ventricular progenitor cells for cardiac repair or to improve cardiac function are also provided. Methods of using the Jagged 1+ and/or Frizzled 4+ ventricular progenitor cells for cardiac toxicity screening of test compounds are also provided.
(FR)
Cette invention concerne Jagged 1 et Frizzled 4 à titre de marqueurs de surface cellulaire pour isoler des cellules progénitrices ventriculaires cardiomyogènes humaines, en particulier des cellules progénitrices qui se différencient préférentiellement en cellules musculaires ventriculaires cardiaques. Par conséquent, l'invention concerne des cellules progénitrices ventriculaires humaines (HVP), et des procédés de séparation in vitro de cellules progénitrices ventriculaires Islet 1+/Jagged 1+ et/ou de cellules progénitrices ventriculaires Islet1+/Frizzled 4+ et/ou de cellules progénitrices ventriculaires Islet 1+/Jagged 1+/Frizzled 4+, et leur expansion et propagation à grande échelle. D'importantes populations clonales de cellules progénitrices ventriculaires Jagged 1+ et/ou Frizzled 4+ isolées sont également décrites. L'invention concerne également des procédés d'utilisation in vivo des cellules progénitrices ventriculaires Jagged 1+ et/ou Frizzled 4+ pour la réparation cardiaque ou pour l'amélioration de la fonction cardiaque. Des procédés d'utilisation des cellules progénitrices ventriculaires Jagged 1+ et/ou Frizzled 4+ pour le criblage de toxicité cardiaque de composés d'essai sont également décrits.
Latest bibliographic data on file with the International Bureau