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1. WO2016028907 - OPTOFLUIDIC SORTER

Publication Number WO/2016/028907
Publication Date 25.02.2016
International Application No. PCT/US2015/045924
International Filing Date 19.08.2015
IPC
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
G01N 21/31 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
CPC
B01L 2200/0668
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0647Handling flowable solids, e.g. microscopic beads, cells, particles
0668Trapping microscopic beads
B01L 2400/0424
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0415electrical forces, e.g. electrokinetic
0424Dielectrophoretic forces
B01L 2400/0454
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0454radiation pressure, optical tweezers
B01L 3/502761
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502761specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G01N 15/1404
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1404Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
Applicants
  • BIO-RAD LABORATORIES, INC. [US]/[US]
Inventors
  • CHU, Daniel Y.
  • PATT, Paul J.
  • TONG, Roger
Agents
  • HINSCH, Matthew E.
Priority Data
62/039,58420.08.2014US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) OPTOFLUIDIC SORTER
(FR) TRIEUSE OPTOFLUIDIQUE
Abstract
(EN)
A method is provided for transporting a plurality of cells through a flow chamber, wherein the cells are initially immobilized on an internal surface of the flow chamber. The method comprises: selectively releasing the cells from the internal surface of the flow chamber; and flowing liquid through the flow chamber such that the released cells travel with the liquid, thereby transporting the cells through the flow chamber. Cells can be immobilized on or selectively released from the internal surface by applying or removing a trapping potential. The trapping potential can arise from an electric field gradient or an optical field gradient. Alternatively, cells can be selectively released from the surface using photocatalysis. Selective release allows cells to be individually observed or analyzed downstream, and can be based on a signal detected from one or more cells immobilized on the surface.
(FR)
La présente invention concerne un procédé pour transporter une pluralité de cellules à travers une chambre d’écoulement, dans lequel des cellules sont initialement immobilisées sur une surface interne de la chambre d’écoulement. Le procédé comprend : la libération sélective des cellules de la surface interne de la chambre d’écoulement ; et l’écoulement de liquide à travers la chambre d’écoulement de sorte que les cellules libérées se déplacent avec le liquide, de manière à transporter les cellules à travers la chambre d’écoulement. Les cellules peuvent être immobilisées sur ou sélectivement libérées de la surface interne par application ou élimination d’un potentiel de piégeage. Le potentiel de piégeage peut être induit par un gradient de champ électrique ou un gradient de champ optique. En variante, les cellules peuvent être sélectivement libérées de la surface au moyen d’une photocatalyse. La libération sélective permet que les cellules soient individuellement observées ou analysées en aval, et peut être basée sur un signal détecté à partir d’une ou plusieurs cellules immobilisées sur la surface.
Also published as
CN201580044435.9
Latest bibliographic data on file with the International Bureau