Processing

Please wait...

Settings

Settings

Goto Application

1. WO2016013880 - USE OF MULTIPLE TARGET NUCLEIC ACID DETECTION METHOD USING CLAMPING PROBE AND DETECTION PROBE

Publication Number WO/2016/013880
Publication Date 28.01.2016
International Application No. PCT/KR2015/007656
International Filing Date 23.07.2015
IPC
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/6818
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6858
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6858Allele-specific amplification
C12Q 1/6886
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
C12Q 2600/106
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
106Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
Applicants
  • 주식회사 파나진 PANAGENE INC. [KR]/[KR]
Inventors
  • 최재진 CHOI, Jae Jin
  • 윤지혜 YOON, Ji Hye
  • 김수남 KIM, Su Nam
  • 김성기 KIM, Sung Kee
  • 김진우 KIM, Jin Woo
Agents
  • 특허법인코리아나 KOREANA PATENT FIRM
Priority Data
10-2014-009505025.07.2014KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) USE OF MULTIPLE TARGET NUCLEIC ACID DETECTION METHOD USING CLAMPING PROBE AND DETECTION PROBE
(FR) UTILISATION DE MULTIPLES PROCÉDÉS DE DÉTECTION D'ACIDE NUCLÉIQUE CIBLE ET SONDE DE DÉTECTION À L'AIDE DE SONDE DE SERRAGE
(KO) 클램핑 프로브 및 검출 프로브를 이용한 다중 표적핵산 검출 방법의 이용
Abstract
(EN)
The present invention relates to an application of a target nucleic acid detection method using a clamping probe and a detection probe. The method of the present invention can effectively detect a small amount of variation or a specific gene sequence contained in a sample by selective amplification and detection of a trace amount of a target gene to be detected while inhibiting amplification of wild-type genes or undesired genes. Also, it is possible to determine a large number of genotypes at the same time through a melting curve analysis. In particular, the method can be used for diagnosis, prognosis and monitoring of the medical condition of a disease, treatment efficacy evaluation, and for aiding nucleic acid and protein delivery studies and so on, through a very small amount of a mutant genotype that is confirmed at a high detection sensitivity. The method of the present invention comprises a step for evaluating the detection of biomarkers such as EGFR, KRAS, NRAS etc. and the presence of mutations of biomarkers using invasive specimens such as tissues as well as non-invasive specimens (blood, urine, sputum, stool, saliva, and cells). The presence of the biomarker and mutations provides a method used for monitoring of the entire cycle of a related disease, disease prognosis and prediction, decision of disease treatment strategy, disease diagnosis/early diagnosis, disease prevention, and development of disease therapeutics.
(FR)
La présente invention concerne une application d'un procédé de détection d'acide nucléique cible à l'aide d'une sonde de serrage et une sonde de détection. Le procédé de la présente invention peut efficacement détecter une petite quantité de variation ou une séquence génique spécifique contenue dans un échantillon par amplification sélective et de détection d'une trace d'un gène cible destiné à être détecté tout en inhibant l'amplification de gènes de type sauvage ou gènes indésirables. De même, il est possible de déterminer un grand nombre de génotypes au même temps à travers une analyse de courbe de fusion. En particulier, le procédé peut être utilisé pour le diagnostic, le pronostic et la surveillance de l'état médical d'une maladie, l'efficacité d'un traitement d'évaluation, et pour aider des études d'acide nucléique et d'administration de protéine et ainsi de suite, à travers une très petite quantité d'un génotype mutant qui est confirmée au niveau d'une sensibilité de détection élevée. Le procédé de la présente invention comprend une étape pour évaluer la détection de biomarqueurs tels qu'EGFR, KRAS, NRAS, etc. et la présence de mutations de biomarqueurs à l'aide de spécimens invasives, telles que des tissus ainsi que des spécimens non-invasif (du sang, de l'urine, du crachat, des selles, de la salive, et des cellules). La présence du biomarqueur et des mutations concerne un procédé utilisé pour la totalité du cycle de surveillance d'une maladie, le pronostic et la prédiction de maladie, la décision de stratégie de traitement d'une maladie, en diagnostic de maladies/diagnostic précoce, la prévention de la maladie, et le développement d'une maladie thérapeutique associés.
(KO)
본 발명은 클램핑 프로브 및 검출 프로브를 이용한 표적핵산 검출방법의 응용에 관한 것으로, 야생형 유전자 또는 원하지 않는 유전자의 증폭을 억제하고 검출하고자 하는 극미량의 표적 유전자의 선택적 증폭 및 검출을 통해 시료 속에 포함된 미량의 변이 또는 특정 유전자 서열을 효과적으로 검출할 수 있다. 또한 융해 곡선 분석을 통한 동시에 다량의 유전형 판별이 가능하다. 특히 높은 검출 민감도로 확인되는 극소량의 돌연변이 유전형을 통해 질환의 의학적 병태를 진단, 예후, 모니터링, 치료 효능의 평가, 핵산 및 단백질 전달연구를 보조하는 방법 등으로 사용 가능하다. 조직과 같은 침습적 검체 뿐 아니라 비침습적 검체(혈액, 소변, 객담, 대변, 타액, 세포)를 이용하여 EGFR, KRAS, NRAS 등의 바이오마커 검출 및 바이오마커의 돌연변이의 존재를 평가하는 단계를 포함하고, 상기 바이오마커 및 돌연변이의 존재는 관련된 질병의 전주기 모니터링, 질병의 예후, 예측, 질병의 치료방침 결정, 질병의 진단/조기진단, 질병의 예방, 질병의 치료제 개발에 이용하기 위한 방법을 제공한다.
Latest bibliographic data on file with the International Bureau