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1. WO2015191626 - QUANTITATIVE PEPTIDE OR PROTEIN ASSAY

Publication Number WO/2015/191626
Publication Date 17.12.2015
International Application No. PCT/US2015/034960
International Filing Date 09.06.2015
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 23/00 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
23Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/-G01N17/178
CPC
G01N 2560/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2560Chemical aspects of mass spectrometric analysis of biological material
G01N 33/6833
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6827Total protein determination, e.g. albumin in urine
683involving metal ions
6833Copper, e.g. Folin-, Lowry-, biuret methods
G01N 33/6839
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6827Total protein determination, e.g. albumin in urine
6839involving dyes, e.g. Coomassie blue, bromcresol green
Applicants
  • PIERCE BIOTECHNOLOGY, INC. [US]/[US]
Inventors
  • HANEY, Jeffrey, Paul
  • ETIENNE, Christopher, L.
  • HOU, Sijian
  • RAJA, Erum
  • GANAPATHY, Ramesh
Agents
  • LYMAN, Beverly, A.
Priority Data
62/010,59411.06.2014US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) QUANTITATIVE PEPTIDE OR PROTEIN ASSAY
(FR) DOSAGE QUANTITATIF DE PEPTIDE OU DE PROTÉINE
Abstract
(EN) Peptide and/or protein quantitation methods, kits, and compositions, particularly useful for mass spectrometry, are provided herein based on a bathocuproine-based composition complex such as bathocuproinedisulfonic acid disodium salt hydrate complex. The methods are one-step rapid absorbance methods using small sample volumes. They produce a robust signal with high signal to background ratio and accurately quantitate even complex peptide mixtures with low variability and high sensitivity.
(FR) La présente invention concerne des procédés, des kits, et des compositions de quantification de peptide et/ou de protéine, particulièrement utiles pour la spectrométrie de masse, basés sur un complexe de composition à base de bathocuproïne tel qu’un complexe de sel disodique d’acide bathocuproïnesulfonique hydraté. Les procédés sont des procédés d’absorbance rapide en une étape utilisant de faibles volumes d’échantillon. Ils génèrent un signal robuste avec un rapport du signal au bruit de fond élevé et quantifient précisément des mélanges de peptides, même complexes, avec une faible variabilité et une haute sensibilité.
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