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1. WO2015145133 - NUCLEIC ACID PREPARATION METHOD

Publication Number WO/2015/145133
Publication Date 01.10.2015
International Application No. PCT/GB2015/050871
International Filing Date 24.03.2015
IPC
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C12N 15/1093
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1093General methods of preparing gene libraries, not provided for in other subgroups
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • CAMBRIDGE ENTERPRISE LIMITED [GB]/[GB]
Inventors
  • BALASUBRAMANIAN, Shankar
  • RAIBER, Eun-Ang
  • MCINROY, Gordon
Agents
  • BARNES, Colin Lloyd
Priority Data
1405226.024.03.2014GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) NUCLEIC ACID PREPARATION METHOD
(FR) PROCÉDÉ DE PRÉPARATION D'ACIDES NUCLÉIQUES
Abstract
(EN) This invention relates to the preparation of nucleic acids, for example bisulfite-treated nucleic acids, for the analysis of modified cytosine marks. Included is a method of preparing a bisulfite treated nucleic acid library comprising a two-step ligation procedure, where a first adapter is added before bisulfite treatment and a second adapter afterwards.
(FR) La présente invention concerne la préparation d'acides nucléiques, par exemple, d'acides nucléiques traités au bisulfite, pour l'analyse de marques portées par des cytosines modifiées. L'invention concerne également un procédé de préparation d'une bibliothèque d'acides nucléiques traités au bisulfite comprenant une procédure de ligature en deux étapes, où un premier adaptateur est ajouté avant un traitement au bisulfite et un second adaptateur est ajouté par la suite.
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