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1. WO2015134523 - CHROMATIN IMMUNOCAPTURE DEVICES AND METHODS OF USE

Publication Number WO/2015/134523
Publication Date 11.09.2015
International Application No. PCT/US2015/018522
International Filing Date 03.03.2015
IPC
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
B01L 2200/0673
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0673Handling of plugs of fluid surrounded by immiscible fluid
B01L 2300/0867
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0867Multiple inlets and one sample wells, e.g. mixing, dilution
B01L 3/502784
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502769characterised by multiphase flow arrangements
502784specially adapted for droplet or plug flow, e.g. digital microfluidics
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Applicants
  • THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS [US]/[US]
  • MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH [US]/[US]
Inventors
  • BAILEY, Ryan
  • TICE, Joshua
  • ORDOG, Tamas
  • LEE, Jeong, Heon
  • GRAYBILL, Richard, Martin
  • XU, Yi
  • DOONAN, Steven
Agents
  • RYBAK, Sheree Lynn
Priority Data
61/947,41503.03.2014US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) CHROMATIN IMMUNOCAPTURE DEVICES AND METHODS OF USE
(FR) DISPOSITIFS D'IMMUNOCAPTURE DE CHROMATINES ET PROCÉDÉS D'UTILISATION
Abstract
(EN) This application provides fluidic devices, such as microfluidic devices, which can be used for the creation and/or manipulation of droplets in droplet-based microfluidic systems, as well as systems and methods for using the same. The microfluidic devices can be used to generate droplets, extract or inject volume to droplets, and/or split droplets. Also provided are methods for generating nucleosomes, and isolated DNA from nucleosomes (or from non- nucleosomes), for example using the disclosed devices.
(FR) La présente invention concerne des dispositifs fluidiques, tels que des dispositifs microfluidiques, qui peuvent être utilisés pour la création et/ou la manipulation de gouttelettes dans des systèmes microfluidiques à base de gouttelettes, ainsi que des systèmes et des procédés pour les utiliser. Les dispositifs microfluidiques peuvent être utilisés pour générer des gouttelettes, extraire ou injecter un volume à des gouttelettes, et/ou diviser des gouttelettes. L'invention concerne également des procédés servant à générer des nucléosomes, et de l'ADN isolé des nucléosomes (ou de non nucléosomes), par exemple à l'aide des dispositifs décrits.
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