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1. WO2015128475 - NOVEL COATED CARRIERS FOR THE SPECIFIC BINDING OF TARGET MOLECULES

Publication Number WO/2015/128475
Publication Date 03.09.2015
International Application No. PCT/EP2015/054164
International Filing Date 27.02.2015
IPC
G01N 33/543 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
A61L 33/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
33Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
A61M 1/36 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
MDEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
1Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
36Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation
A61L 2/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
CPC
A61L 33/0011
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
33Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
0005Use of materials characterised by their function or physical properties
0011Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
A61M 1/36
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
MDEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY
1Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
36Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; ; Extra-corporeal blood circuits
A61M 1/3679
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
MDEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY
1Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
36Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; ; Extra-corporeal blood circuits
3679by absorption
G01N 33/54393
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54393Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Applicants
  • LEUKOCARE AG [DE]/[DE]
Inventors
  • KIRCHNER, Roland
  • ALTRICHTER, Jens
  • SCHOLZ, Martin
Agents
  • VOSSIUS & PARTNER
Priority Data
14157002.827.02.2014EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) NOVEL COATED CARRIERS FOR THE SPECIFIC BINDING OF TARGET MOLECULES
(FR) NOUVEAUX PORTEURS REVÊTUS POUR LA LIAISON SPÉCIFIQUE DE MOLÉCULES CIBLES
Abstract
(EN) The present invention relates to a method of producing a solid carrier, to which at least one affinity-reagent is coupled that binds specifically to a target molecule, comprising the steps of (i) coupling at least one affinity-reagent to a solid carrier, (ii) overlaying the affinity-reagent-coupled carrier obtained in (i) by incubation with a composition comprising heparin and/or a functional derivative thereof, and (iii) overlaying the affinity-reagent-coupled carrier by incubation with a composition comprising stabilising excipients, wherein steps (ii) and (iii) are carried out subsequently or simultaneously and subsequently to step (i). The invention further relates to a solid carrier comprising (i) at least one affinity-reagent, wherein the at least one affinity-reagent is coupled to the solid carrier, (ii) an overlay comprising heparin and/or a functional derivative thereof, and (ii) an overlay comprising stabilising excipients. In addition, the invention relates to the use of the carrier of the invention or of the carrier produced by the method of the invention to specifically bind and enrich a target molecule or a target cell which carries a biological target molecule on its surface from a liquid sample. Further, the invention relates to the use of the carrier of the invention or of the carrier produced by the method of the invention to specifically induce a biological response in a target cell. Furthermore, the invention relates to an ex-vivo or in-vitro method for specifically binding and enriching a target cell out of a liquid sample comprising contacting the liquid sample with the carrier of the invention or the carrier produced by the method of the invention, wherein the contacting is effected by inducing the liquid sample to flow over the surface of the carrier to which the at least one affinity-reagent is coupled, and wherein the target cell carries a biological target molecule on its surface to which the at least one affinity-reagent specifically binds. Moreover, the invention relates to the carrier of the invention or the carrier produced by the methods of the invention for use as an implant or as an extracorporeal device.
(FR) La présente invention porte sur un procédé de production d'un porteur solide, auquel au moins un réactif d'affinité est couplé, ce dernier se liant de façon spécifique à une molécule cible, lequel procédé comprend les étapes consistant à (i) coupler au moins un réactif d'affinité à un porteur solide, (ii) recouvrir le porteur couplé à un réactif d'affinité obtenu en (i) par incubation avec une composition comprenant de l'héparine et/ou un dérivé fonctionnel de cette dernière, et (iii) recouvrir le porteur couplé à un réactif d'affinité par incubation avec une composition comprenant des excipients stabilisants, les étapes (ii) et (iii) étant effectuées successivement ou simultanément, et après l'étape (i). L'invention porte également sur un porteur solide comprenant (i) au moins un réactif d'affinité, le ou les réactifs d'affinité étant couplés au porteur solide, (ii) un revêtement comprenant de l'héparine et/ou un dérivé fonctionnel de cette dernière, et (iii) un revêtement comprenant des excipients stabilisants. L'invention porte également sur l'utilisation du porteur selon l'invention ou du porteur produit par le procédé selon l'invention pour se lier de façon spécifique à une molécule cible ou à une cellule cible qui porte une molécule cible biologique sur sa surface à partir d'un échantillon liquide, et enrichir cette dernière. L'invention porte également sur un procédé ex vivo ou in vitro pour se lier de façon spécifique à une cellule cible à partir d'un échantillon liquide et enrichir cette dernière, lequel procédé met en œuvre la mise en contact de l'échantillon liquide avec le porteur selon l'invention ou le porteur produit par le procédé selon l'invention, la mise en contact étant effectuée par le fait d'induire l'échantillon liquide à s'écouler sur la surface du porteur à laquelle sont couplés le ou les réactifs d'affinité, et la cellule cible portant une molécule cible biologique sur sa surface, à laquelle se lient de façon spécifique le ou les réactifs d'affinité. L'invention porte également sur le porteur selon l'invention ou le porteur produit par les procédés selon l'invention pour l'utilisation comme implant ou comme dispositif extracorporel.
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