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1. WO2015128448 - LASER MICRODISSECTION SYSTEM AND LASER MICRODISSECTION METHOD

Publication Number WO/2015/128448
Publication Date 03.09.2015
International Application No. PCT/EP2015/054103
International Filing Date 27.02.2015
IPC
G01N 1/28 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
CPC
G01N 1/286
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
286involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
G01N 2001/284
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
2833Collecting samples on a sticky, tacky, adhesive surface
284using local activation of adhesive, i.e. Laser Capture Microdissection
G01N 2001/2886
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
286involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
2873Cutting or cleaving
2886Laser cutting, e.g. tissue catapult
G02B 21/24
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
24Base structure
G02B 21/32
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
32Micromanipulators structurally combined with microscopes
Applicants
  • LEICA MICROSYSTEMS CMS GMBH [DE]/[DE]
Inventors
  • SCHLAUDRAFF, Falk
Agents
  • BRADL, Joachim
Priority Data
10 2014 203 656.928.02.2014DE
Publication Language German (de)
Filing Language German (DE)
Designated States
Title
(DE) LASERMIKRODISSEKTIONSSYSTEM UND LASERMIKRODISSEKTIONSVERFAHREN
(EN) LASER MICRODISSECTION SYSTEM AND LASER MICRODISSECTION METHOD
(FR) SYSTÈME ET PROCÉDÉ DE MICRODISSECTION LASER
Abstract
(DE) Lasermikrodissektionsverfahren zum Gewinnen eines Probenbereichs (510) aus einer biologischen Probe (51), bei dem ein fokussierter Laserstrahl (77) entlang zumindest eines Teils einer den Probenbereich (510) umgebenden Schnittlinie (550) geführt wird, wodurch der Probenbereich (510) von der umgebenden Probe (51) getrennt wird, und wobei vor einem Ablösen des Probenbereichs (510) mindestens zwei Stege (560) verbleiben, die den Probenbereich (510) mit der umgebenden Probe (51) verbinden, wobei mindestens ein weiterer auf die Schnittlinie fokussierter Laserstrahl (77', 77") bereitgestellt wird, und dass jeder der mindestens zwei verbleibenden Stege (560) durch jeweils einen der Laserstrahlen (77, 77', 77") durchtrennt wird.
(EN) Laser microdissection method for obtaining a sample region (510) from a biological sample (51), wherein a focused laser beam (77) is guided along at least one part of a cutting line (550) surrounding the sample region (510), by which means the sample region (510) is separated from the surrounding sample (51), and wherein at least two bridges (560) remain prior to a release of the sample region (560), which bridges connect the sample region (510) to the surrounding sample (51), wherein at least one further laser beam (77’, 77’’) focused on the cutting line is provided, and each of the at least two remaining bridges (560) is severed by, in each case, one of the laser beams (77, 77', 77").
(FR) L'invention concerne un procédé de microdissection laser, servant à prélever une zone (510) d'un échantillon biologique (51), dans lequel on guide un rayon laser (77) concentré le long d'une partie au moins d'une ligne de coupe (550) entourant la zone (510) de l'échantillon afin de séparer ladite zone (510) de l'échantillon (51) environnant. Avant de détacher la zone (510) de l'échantillon, on laisse au moins deux pontets (560) qui relient la zone (510) à l'échantillon (51) environnant. Il est prévu au moins un autre rayon laser (77', 77") concentré sur la ligne de coupe et chacun desdits au moins deux pontets (560) subsistants est sectionné respectivement par un des rayons laser (77, 77', 77").
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