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1. (WO2015127365) CALCIUM-INDEPENDENT SORTASE A MUTANTS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2015/127365    International Application No.:    PCT/US2015/017116
Publication Date: 27.08.2015 International Filing Date: 23.02.2015
IPC:
C12N 9/52 (2006.01)
Applicants: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH [US/US]; Nine Cambridge Center Cambridge, MA 02142 (US)
Inventors: INGRAM, Jessica; (US).
PLOEGH, Hidde; (US)
Agent: WARREN, Lisa, M.; (US)
Priority Data:
61/943,042 21.02.2014 US
Title (EN) CALCIUM-INDEPENDENT SORTASE A MUTANTS
(FR) MUTANTS DE SORTASE A CALCIUM-INDÉPENDANTS
Abstract: front page image
(EN)Disclosed herein are calcium-independent sortase A mutants exhibiting increased catalytic activity compared to wild-type sortase A. In some aspects, the disclosure provides a sortase A mutant comprising at least three amino acid substitutions relative to a wild-type sortase A, wherein the amino acid substitutions comprise a) a K residue at position 105; b) a Q or A residue at position 108; and c) at least one amino acid substitution selected from the group consisting of i) a R residue at position 94; ii) a S residue at position 94; iii) a N residue at position.160; iv) a A residue at position 165; v) a E residue at 5 position 190; and vi) a T residue at position 196. In some embodiments, the mutant comprises a deletion of amino acids 2-25.
(FR)Cette invention concerne des mutants de sortase A calcium-indépendants manifestant une activité catalytique accrue comparativement à la sortase A de type sauvage.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)