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1. (WO2015127277) METHOD FOR GUIDING CELL SPREADING IN AUTOMATED CYTOGENETIC ASSAYS

Pub. No.:    WO/2015/127277    International Application No.:    PCT/US2015/016919
Publication Date: Fri Aug 28 01:59:59 CEST 2015 International Filing Date: Sat Feb 21 00:59:59 CET 2015
IPC: G01N 21/45
Applicants: THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
Inventors: ZENHAUSERN, Frederic
GU, Jian
Title: METHOD FOR GUIDING CELL SPREADING IN AUTOMATED CYTOGENETIC ASSAYS
Abstract:
Provided herein are methods and related systems for controlling droplet spreading on a surface, including droplets in which a biological component is suspended. A biological solution is provided as a droplet to a surface. Interference fringes generated by the droplet on the surface are imaged, wherein the imaging is over a time course during which the droplet spreads on the surface. A droplet parameter is determined from the imaging step and a process parameter controlled to obtain an interference fringe pattern corresponding to a desired droplet parameter. In this manner, well-controlled droplet spreading is achieved, which is important in a range of applications, including assays that rely on good metaphase spreading.