Search International and National Patent Collections

1. (WO2015126078) METHOD FOR DETECTING NUCLEIC ACID USING ASYMMETRIC ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID AND SIGNAL PROBE

Pub. No.:    WO/2015/126078    International Application No.:    PCT/KR2015/001070
Publication Date: Fri Aug 28 01:59:59 CEST 2015 International Filing Date: Tue Feb 03 00:59:59 CET 2015
IPC: C12Q 1/68
Applicants: DXGENE, INC
디엑스진주식회사
Inventors: KIM, MinHwan
김민환
Title: METHOD FOR DETECTING NUCLEIC ACID USING ASYMMETRIC ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID AND SIGNAL PROBE
Abstract:
The present invention relates to a method for accurately detecting a target nucleic acid by asymmetrically and isothermally amplifying the target nucleic acid using an external primer set, an internal primer set having different percentages of forward and reverse DNA-RAN-DNA hybrid primers, and a DNA-RNA-DNA hybrid signal probe and amplifying a signal of the probe at the same time. According to the present invention, the signal of the probe can be efficiently amplified compared with the method of the prior art, a symmetric iTPA method, which is an isothermal primer and probe amplification method using the same percentage of primers. Therefore, the present invention is applied to the accurate detection and confirmation of pathogens, detection of gene modification inducing identified phenotypes, diagnosis of susceptibility to genetic diseases, evaluation of gene expression, and various genome projects, and thus is useful in the molecular biological researches and disease diagnosis.