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1. (WO2015126033) COMPOSITION FOR CONTROLLING STEM CELL PLURIPOTENCY, CONTAINING LIN28A METHYLATION INHIBITOR, AND METHOD FOR SCREENING FOR LIN28A METHYLATION INHIBITOR
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2015/126033    International Application No.:    PCT/KR2014/010410
Publication Date: 27.08.2015 International Filing Date: 03.11.2014
IPC:
C12N 5/02 (2006.01), C12N 15/113 (2010.01), C12N 5/0735 (2010.01), A61P 35/00 (2006.01)
Applicants: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY [KR/KR]; 291 Daehak-ro Yuseong-gu Daejeon 305-338 (KR)
Inventors: LEE, Daeyoup; (KR).
KIM, Seung-Kyoon; (KR).
LEE, Hosuk; (KR)
Agent: LEE, Cheo Young; (KR)
Priority Data:
10-2014-0019261 19.02.2014 KR
Title (EN) COMPOSITION FOR CONTROLLING STEM CELL PLURIPOTENCY, CONTAINING LIN28A METHYLATION INHIBITOR, AND METHOD FOR SCREENING FOR LIN28A METHYLATION INHIBITOR
(FR) COMPOSITION POUR RÉGULER LA PLURIPOTENCE DE CELLULES SOUCHES, CONTENANT UN INHIBITEUR DE LA MÉTHYLATION DU LIN28A, ET PROCÉDÉ DE CRIBLAGE POUR UN INHIBITEUR DE LA MÉTHYLATION DU LIN28A
(KO) LIN28A 메틸화 억제제를 포함하는 줄기세포 전능화 조절용 조성물 및 LIN28A 메틸화 억제제의 스크리닝 방법
Abstract: front page image
(EN)The present invention relates to: a composition for controlling stem cell pluripotency, containing an LIN28A methylation inhibitor; and a method for screening for an LIN28A methylation inhibitor, and more particularly, to a composition for controlling embryonic stem cell pluripotency or an anticancer composition, which is a composition for controlling stem cell pluripotency or treating cancer, containing an inhibitor for controlling the methylation of the 135th lysine of LIN28A to be methylated by Set7/9, and a method for screening for the inhibitor, wherein the screening method comprises the steps of: (a) making a candidate material come into contact with cells into which the LIN28A gene is introduced; (b) measuring the methylation level of the 135th lysine of LIN28A; and (c) selecting an inhibitor for controlling the methylation of the 135th lysine of LIN28A. The method of the present invention can effectively screen for a material capable of controlling embryonic stem cell pluripotency or a material having an anticancer activity, and the inhibitor screened for by the method of the present invention controls embryonic stem cell pluripotency, and is thus effective in the preparation of an agent for treating stem cell differentiation disorders or cancer by inhibiting the growth of cancer cells.
(FR)La présente invention concerne une composition pour réguler la pluripotence de cellules souches, contenant un inhibiteur de la méthylation du LIN28A ; et un procédé de criblage portant sur un inhibiteur de la méthylation du LIN28A, et plus particulièrement une composition pour réguler la pluripotence de cellules souches embryonnaires ou une composition anticancéreuse, qui est une composition pour réguler la pluripotence de cellules souches ou pour traiter un cancer, contenant un inhibiteur destiné à réguler la méthylation de la 135ème lysine de LIN28A à méthyler par Set7/9, et un procédé pour un criblage portant sur l'inhibiteur, le procédé de criblage comprenant les étapes de : (a) mise en contact d'un matériau candidat avec des cellules dans lesquelles le gène LIN28A est introduit ; (b) mesure du niveau de méthylation de la 135ème lysine de LIN28A ; et (c) sélection d'un inhibiteur pour réguler la méthylation de la 135ème lysine de LIN28A. Le procédé de la présente invention peut efficacement cribler pour un matériau capable de réguler la pluripotence de cellules souches embryonnaires ou un matériau ayant une activité anticancéreuse, et l'inhibiteur criblé par le procédé de la présente invention régule la pluripotence des cellules souches embryonnaires et est ainsi efficace pour la préparation d'un agent destiné au traitement des troubles de la différenciation des cellules souches ou d'un cancer par inhibition de la croissance de cellules cancéreuses.
(KO)본 발명은 LIN28A 메틸화 억제제를 포함하는 줄기세포 전능화 조절용 조성물 및 LIN28A 메틸화 억제제를 스크리닝하는 방법에 관한 것으로, 보다 구체적으로는 Set7/9에 의해 메틸화 되는 LIN28A 135번째 라이신의 메틸화를 제어하는 억제제를 포함하는 줄기세포 전능화 조절용 또는 암 치료용 조성물 및 상기 억제제를 스크리닝하는 방법으로, 상기 스크리닝 방법은 (a) 후보물질을 LIN28A 유전자가 도입된 세포에 접촉시키는 단계 (b) LIN28A의 135번째 라이신의 메틸화 수준을 측정하는 단계 및 (c) LIN28A의 135번째 라이신의 메틸화를 제어하는 억제제를 선택하는 단계를 포함하는 배아줄기세포 전분화능 조절용 조성물 또는 항암 조성물 및 스크리닝 방법에 관한 것이다. 본 발명의 방법은 배아줄기세포의 전분화능을 조절할 수 있는 물질 또는 항암활성이 있는 물질을 효과적으로 스크리닝 할 수 있으며, 본 발명의 방법에 의해 스크리닝된 억제제는 배아줄기세포의 전분화능을 조절하고, 암세포의 성장을 억제하여 줄기세포 분화 장애 또는 암 치료제 제조에 효과적이다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)