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1. (WO2015125662) METHOD FOR PROMOTING DIFFERENTIATION OF STEM CELLS USING AMINO ACID REFORMULATED MEDIUM, STEM CELLS PROCESSED USING SAID METHOD, AND MEDIUM
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2015/125662 International Application No.: PCT/JP2015/053607
Publication Date: 27.08.2015 International Filing Date: 10.02.2015
Chapter 2 Demand Filed: 17.12.2015
IPC:
C12N 5/071 (2010.01) ,C12N 5/0735 (2010.01) ,C12N 5/10 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
国立大学法人熊本大学 NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY [JP/JP]; 熊本県熊本市中央区黒髪二丁目39番1号 2-39-1, Kurokami, Chuo-ku, Kumamoto-shi Kumamoto 8608555, JP
味の素株式会社 AJINOMOTO CO., INC. [JP/JP]; 東京都中央区京橋1丁目15番1号 15-1, Kyobashi 1-chome, Chuo-ku Tokyo 1048315, JP
Inventors:
粂 昭苑 KUME, Shoen; JP
遠藤 文夫 ENDO, Fumio; JP
白木 伸明 SHIRAKI, Nobuaki; JP
白木 恭子 SHIRAKI, Yasuko; JP
粂 和彦 KUME, Kazuhiko; JP
馬渡 一徳 MAWATARI, Kazunori; JP
Agent:
大澤 健一 OSAWA Kenichi; JP
Priority Data:
2014-03206821.02.2014JP
2014-18429410.09.2014JP
Title (EN) METHOD FOR PROMOTING DIFFERENTIATION OF STEM CELLS USING AMINO ACID REFORMULATED MEDIUM, STEM CELLS PROCESSED USING SAID METHOD, AND MEDIUM
(FR) PROCÉDÉ DE STIMULATION DE LA DIFFÉRENCIATION DES CELLULES SOUCHES AU MOYEN D’UN MILIEU REFORMULÉ CONTENANT DES ACIDES AMINÉS, CELLULES SOUCHES TRAITÉES À L'AIDE DUDIT PROCÉDÉ, ET MILIEU
(JA) アミノ酸組成変更培地を用いた幹細胞の分化促進方法、及び該方法を用いて処理された幹細胞、並びに培地
Abstract:
(EN) The objective of the present invention is to provide a medium and a method for improving the rate of induced differentiation of stem cells such as ES cells or iPS cells. By means of the present invention, it is possible to efficiently induce differentiation by means of culturing stem cells in an undifferentiation maintaining medium from which methionine has been eliminated before inducing differentiation by culturing in a differentiation inducing medium. The present invention provides a method that is for inducing differentiation of mammal-derived pluripotent stem cells and that contains: a step for culturing the pluripotent stem cells in an undifferentiation maintaining medium for pluripotent stem cells and comprising a medium not containing methionine; and a step for culturing the pluripotent stem cells in a differentiation inducing medium to induce differentiation. The present invention also provides pluripotent stem cells cultured by means of the undifferentiation maintaining medium for mammal-derived pluripotent stem cells and comprising a medium not containing methionine. The present invention further provides an undifferentiation maintaining medium that is for pluripotent stem cells and does not contain methionine in the medium.
(FR) La présente invention a pour objet un milieu et un procédé d’amélioration de la vitesse de différenciation induite des cellules souches telles que des cellules ES ou des cellules iPS. Au moyen de la présente invention, il est possible d'induire efficacement la différenciation par le biais de la mise en culture des cellules souches dans un milieu maintenant la non-différentiation dont la méthionine a été éliminée avant induction de la différenciation par mise en culture dans un milieu induisant la différenciation. La présente invention concerne un procédé d’induction de la différenciation de cellules souches pluripotentes dérivées de mammifères qui contient : une étape de culture des cellules souches pluripotentes dans un milieu de maintien de la non-différentiation des cellules souches pluripotentes et comprenant un milieu ne contenant pas de méthionine ; et une étape de mise en culture des cellules souches pluripotentes dans un milieu d'induction de différenciation pour induire la différenciation. La présente invention concerne également des cellules souches pluripotentes mises en culture au moyen du milieu maintenant la non-différentiation des cellules souches pluripotentes dérivées de mammifères et comprenant un milieu ne contenant pas de méthionine. La présente invention concerne en outre un milieu maintenant la non-différentiation des cellules souches pluripotentes et ne contenant pas de méthionine.
(JA)  本発明は、ES細胞やiPS細胞などの幹細胞の分化誘導効率を改善する方法及び培地を提供することを目的とする。本発明により、幹細胞を、分化誘導培地で培養して分化誘導する前に、メチオニンを除去した未分化維持培地で培養することにより、効率よく分化誘導できる。本発明は、哺乳動物由来の多能性幹細胞を分化誘導する方法であって、該多能性幹細胞を、メチオニンを含まない培地からなる多能性幹細胞の未分化維持培地で培養する工程、および、該多能性幹細胞を、分化誘導培地で培養して分化誘導する工程、を含む分化誘導方法を提供する。本発明はまた、メチオニンを含まない培地からなる哺乳動物由来の多能性幹細胞の未分化維持培地で培養された多能性幹細胞を提供する。本発明はさらには、培地中にメチオニンを含まない多能性幹細胞の未分化維持培地を提供する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)