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1. (WO2015120445) ISOLATION OF CELL-FREE NUCLEIC ACIDS FROM BODILY FLUID SAMPLES USING SOLID CHAOTROPIC AGENTS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2015/120445 International Application No.: PCT/US2015/015191
Publication Date: 13.08.2015 International Filing Date: 10.02.2015
IPC:
C12N 15/10 (2006.01)
Applicants: QUAN, Nancy; US
Inventors: QUAN, Nancy; US
Agent: FOLEY, Shawn; US
Priority Data:
61/937,84410.02.2014US
Title (EN) ISOLATION OF CELL-FREE NUCLEIC ACIDS FROM BODILY FLUID SAMPLES USING SOLID CHAOTROPIC AGENTS
(FR) ISOLEMENT D'ACIDES NUCLÉIQUES ACELLULAIRES À PARTIR D'ÉCHANTILLONS DE FLUIDES CORPORELS ET À L'AIDE D'AGENTS CHAOTROPIQUES SOLIDES
Abstract: front page image
(EN) Disclosed is a process for isolating cell free nucleic acid (including both DNA and RNA) or an analog thereof from a bodily fluid, and which entails: a) mixing in a container the bodily fluid, a chaotropic agent in solid form, a detergent and a buffer, and a solid phase which includes magnetic particles, thus forming a reaction mixture containing the cell free nucleic acid; b) magnetically separating the solid phase having the cell free nucleic acid bound thereto from the reaction mixture; and optionally c) dissociating the nucleic acid from the solid phase. Compositions and kits are also disclosed.
(FR) L'invention concerne un procédé d'isolement d'acides nucléiques acellulaires (ADN ou ARN) ou d'analogues de ceux-ci à partir d'un fluide corporel. Ledit procédé consiste : a) à mélanger dans un récipient le fluide corporel, un agent chaotropique sous forme solide, un détergent et un tampon, et une phase solide comprenant des particules magnétiques, de façon à obtenir un mélange réactionnel contenant l'acide nucléique acellulaire ; b) à séparer magnétiquement la phase solide à laquelle est lié l'acide nucléique acellulaire du mélange réactionnel ; et, éventuellement, c) à dissocier l'acide nucléique de la phase solide. L'invention concerne également des compositions et des kits.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)