Search International and National Patent Collections

1. (WO2015120223) METHOD FOR SEPARATING HYALURONAN AND QUANTIFYING ITS MOLECULAR MASS DISTRIBUTION IN BIOLOGICAL SAMPLES

Pub. No.:    WO/2015/120223    International Application No.:    PCT/US2015/014742
Publication Date: Fri Aug 14 01:59:59 CEST 2015 International Filing Date: Sat Feb 07 00:59:59 CET 2015
IPC: C12P 19/26
Applicants: NEW YORK UNIVERSITY
Inventors: COWMAN, Mary
YUAN, Han
AMIN, Ripal
Title: METHOD FOR SEPARATING HYALURONAN AND QUANTIFYING ITS MOLECULAR MASS DISTRIBUTION IN BIOLOGICAL SAMPLES
Abstract:
The present invention provides a facile method for separation (fractionation) of HA in a sample over a broad M range, including low M HA, by ion exchange (IEX) chromatography. The present invention also provides an assay method for quantifying in a sample the presence of low M HA in total HA isolated from a biological source. The method involves HA fractionation according to M by use of IEX separation, followed by HA-specific assay of HA size range fractions.