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1. (WO2015120223) METHOD FOR SEPARATING HYALURONAN AND QUANTIFYING ITS MOLECULAR MASS DISTRIBUTION IN BIOLOGICAL SAMPLES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2015/120223    International Application No.:    PCT/US2015/014742
Publication Date: 13.08.2015 International Filing Date: 06.02.2015
IPC:
C12P 19/26 (2006.01)
Applicants: NEW YORK UNIVERSITY [US/US]; 70 Washington Square New York, New York 10012 (US)
Inventors: COWMAN, Mary; (US).
YUAN, Han; (US).
AMIN, Ripal; (IN)
Agent: BROWDY, Roger, L.; BROWDY AND NEIMARK, PLLC 1625 K Street, NW Suite 1100 Washington, District of Columbia 20006 (US)
Priority Data:
61/936,503 06.02.2014 US
62/100,654 07.01.2015 US
Title (EN) METHOD FOR SEPARATING HYALURONAN AND QUANTIFYING ITS MOLECULAR MASS DISTRIBUTION IN BIOLOGICAL SAMPLES
(FR) PROCÉDÉ DE SÉPARATION DE HYALURONAN ET DE QUANTIFICATION DE SA DISTRIBUTION MOLÉCULAIRE MASSIQUE DANS DES ÉCHANTILLONS BIOLOGIQUES
Abstract: front page image
(EN)The present invention provides a facile method for separation (fractionation) of HA in a sample over a broad M range, including low M HA, by ion exchange (IEX) chromatography. The present invention also provides an assay method for quantifying in a sample the presence of low M HA in total HA isolated from a biological source. The method involves HA fractionation according to M by use of IEX separation, followed by HA-specific assay of HA size range fractions.
(FR)La présente invention concerne un procédé simple de séparation (fractionnement) de HA dans un échantillon sur un large spectre de masse moléculaire (M), comprenant HA de faible masse moléculaire (M), par chromatographie d'échange d'ions (CEI). La présente invention concerne également une méthode de dosage pour quantifier dans un échantillon la présence de HA de faible masse moléculaire (M) dans HA total isolé d'une source biologique. Le procédé comprend le fractionnement de HA conformément à M par séparation par CEI, suivi du dosage spécifique de HA des fractions granulométriques de HA.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)