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1. (WO2015117431) SITE-DIRECTED MUTAGENETIC LAMBDA PHAGE INT RECOMBINANT PROTEIN AND PREPARATION METHOD THEREFOR

Pub. No.:    WO/2015/117431    International Application No.:    PCT/CN2014/090374
Publication Date: Fri Aug 14 01:59:59 CEST 2015 International Filing Date: Thu Nov 06 00:59:59 CET 2014
IPC: C12N 9/50
C12N 15/57
C12N 15/70
Applicants: CYAGEN BIOSCIENCES (GUANGZHOU) INC
赛业(广州)生物科技有限公司
Inventors: ZHANG, Wei
张玮
WEN, Huajie
温华杰
LI, Chunyuan
李春园
MENG, Weineng
蒙伟能
WANG, Wenzhong
王文忠
SHI, Jinxiu
施金秀
Title: SITE-DIRECTED MUTAGENETIC LAMBDA PHAGE INT RECOMBINANT PROTEIN AND PREPARATION METHOD THEREFOR
Abstract:
Provided are a site-directed mutagenetic lambda phase int recombinant protein and a preparation method therefor. The recombinant protein is a lambda phage int recombinant protein with mutations in G347H and D351H, is a recombinant protein that is temperature-induced in terms of activity, where no DNA recombination reaction is started in a low-temperature condition, and a DNA recombination reaction is started at a certain temperature condition, thus allowing for control of the DNA recombination reaction. The recombinant protein is applicable in launching a large sample recombination reaction, thus increasing recombinant sample throughput.