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|1. (WO2015117431) SITE-DIRECTED MUTAGENETIC LAMBDA PHAGE INT RECOMBINANT PROTEIN AND PREPARATION METHOD THEREFOR|
|Applicants:||CYAGEN BIOSCIENCES (GUANGZHOU) INC
|Title:||SITE-DIRECTED MUTAGENETIC LAMBDA PHAGE INT RECOMBINANT PROTEIN AND PREPARATION METHOD THEREFOR|
Provided are a site-directed mutagenetic lambda phase int recombinant protein and a preparation method therefor. The recombinant protein is a lambda phage int recombinant protein with mutations in G347H and D351H, is a recombinant protein that is temperature-induced in terms of activity, where no DNA recombination reaction is started in a low-temperature condition, and a DNA recombination reaction is started at a certain temperature condition, thus allowing for control of the DNA recombination reaction. The recombinant protein is applicable in launching a large sample recombination reaction, thus increasing recombinant sample throughput.