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1. (WO2015117093) EXPRESSION AND PURIFICATION OF CRM197 AND RELATED PROTEINS

Pub. No.:    WO/2015/117093    International Application No.:    PCT/US2015/014130
Publication Date: Fri Aug 07 01:59:59 CEST 2015 International Filing Date: Tue Feb 03 00:59:59 CET 2015
IPC: C12N 15/64
C12N 15/00
C12N 5/00
C12N 5/02
C12P 21/06
Applicants: FINA BIOSOLUTIONS, LLC
Inventors: OGANESYAN, Natalia
LEES, Andrew
Title: EXPRESSION AND PURIFICATION OF CRM197 AND RELATED PROTEINS
Abstract:
The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.