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1. (WO2015116779) ECONOMICAL MOLECULES FOR SPECIFIC BINDING AND DETECTION OF NUCLEIC ACIDS USING UNIVERSAL FUNCTIONALIZED STRANDS

Pub. No.:    WO/2015/116779    International Application No.:    PCT/US2015/013450
Publication Date: Fri Aug 07 01:59:59 CEST 2015 International Filing Date: Fri Jan 30 00:59:59 CET 2015
IPC: C12Q 1/68
Applicants: WILLIAM MARSH RICE UNIVERSITY
Inventors: ZHANG, David, Yu
WANG, Juexiao
Title: ECONOMICAL MOLECULES FOR SPECIFIC BINDING AND DETECTION OF NUCLEIC ACIDS USING UNIVERSAL FUNCTIONALIZED STRANDS
Abstract:
Nucleic acid probes are described comprising a universal component and a target dependent component. The universal component provides an economical advantage in that the universal component can retain any desired functional moiety, such as a fluorophore or other label which can be used with any target dependent component. Thus, the cost of designing target specific functionalized probes is significantly reduced with this probe system by limiting de novo synthesis to only the target dependent component of the probe for each desired target nucleic acid. Furthermore, the probe design of the present disclosure provides high target specificity demonstrating selective binding of the target in a 1% target load sample.