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1. (WO2015069796) METHODS FOR ENGINEERING SUGAR TRANSPORTER PREFERENCES
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WHAT IS CLAIMED IS:

1. A recombinant xylose transporter protein comprising a transporter motif sequence corresponding to amino acid residue positions 36, 37, 38, 39, 40, and 41 oi Candida intermedia GXSl protein, wherein said transporter motif sequence is -G-G/F-X1-X2-X3-G-;

wherein,

X1 is D, C, G, H, I, L, or F;

X2 is A, D, C, E, G, H, or I;

X3 is N, C, Q, F, G, L, M, S, T, or P; and

wherein, said transporter motif sequence is not -G-G-L-I-F-G- or -G-G-F-I-F-G-.

2. The recombinant xylose transporter protein of claim 1, wherein,

X1 is D, C, G, H, or F;

X2 is H or I; and

X3 is S, T, or M.

3. The recombinant xylose transporter protein of claim 1, wherein X1 is F, X2 is I, and X3 is M or S.

4. The recombinant xylose transporter protein of claim 1, wherein said transporter motif sequence is -G-G-F-I-M-G-, -G-F-F-I-M-G-, -G-G-F-I-S-G-, -G-F-F-I-S-G-, -G-G-F-I-T-G-, -G-F-F-I-T-G-, -G-G-F-L-M-G-, -G-F-F-L-M-G-, -G-G-F-L-S-G-,

-G-F-F-L-S-G-, -G-G-F-L-T-G-, -G-F-F-L-T-G-, -G-G-F-H-M-G-, -G-F-F-H-M-G-,

-G-G-F-H-S-G-, -G-F-F-H-S-G-, -G-G-F-H-T-G- or -G-F-F-H-T-G-.

5. The recombinant xylose transporter protein of claim 4, wherein said transporter motif sequence is -G-G-F-I-M-G-, -G-F-F-I-M-G-, -G-G-F-I-S-G-, or -G-F-F-I-S-G-.

6. The recombinant xylose transporter protein of claim 5 further comprising a mutation of an amino acid at the residue position corresponding to 297 of Candida intermedia GXSl protein.

7. The recombinant xylose transporter protein of claim 6, wherein said amino acid at the residue position corresponding to 297 of Candida intermedia GXSl protein is substituted with a Met, Ala, Ser, or Asn residue.

8. The recombinant xylose transporter protein of claim 7, wherein said recombinant xylose transporter protein is derived from a C. intermedia gxsl transporter protein, a S. stipitis rgt2 transporter protein, or a S. cerevisiae hxtl transporter protein.

9. A recombinant galactose-arabinose transporter protein comprising a transporter motif sequence corresponding to amino acid residue positions 36, 37, 38, 39, 40, and 41 of Candida intermedia GXS1 protein, wherein said transporter motif sequence is -G-G/F-X4-X5-X6-G-;

wherein,

X4 is D, C, F, G, H, L, R, T, or P;

X5 is A, C, E, F, H, K, S, P, or V;

X6 is R, D, E, F, H, I, M, T, or Y; and

wherein said sequence is not -G-G-L-V-Y-G-, or -G-G-F-V-F-G-.

10. The recombinant galactose-arabinose transporter protein of claim 9, wherein,

X4 is R, T, H, or F;

X5 is P, H, or V; and

X6 is T, H, F, M, or Y.

1 1. The recombinant galactose-arabinose transporter protein of claim 9, wherein

X4 is F or T;

X5 is P or I; and

X6 is M or T.

12. The recombinant galactose-arabinose transporter protein of claim 1 1, wherein said transporter motif sequence is -G-G-F-H-M-G-, -G-F-F-H-M-G-,

-G-G-R-P-T-G-, -G-F-R-P-T-G-, -G-G-T-P-T-G-, or -G-F-T-P-T-G-.

13. The recombinant galactose-arabinose transporter protein of claim 12, wherein said galactose-arabinose transporter protein further comprises a mutation of an amino acid at the residue position corresponding to 297 of Candida intermedia GXS1 protein.

14. The recombinant galactose-arabinose transporter protein of claim 13, wherein said amino acid at the residue position corresponding to 297 of Candida intermedia GXS1 protein is substituted with a Met, Thr, Ala, or He residue.

15. The recombinant galactose-arabinose transporter protein of claim 14, wherein said recombinant galactose-arabinose transporter protein is derived from a C. intermedia gxsl transporter protein, a S. stipitis rgt2 transporter protein, a S. cerevisiae hxtl transporter protein, or a S. cerevisiae GAL2 protein.

16. A yeast cell comprising the recombinant xylose transporter protein of claim 1.

17. A yeast cell comprising the recombinant galactose-arabinose transporter protein of claim 9.

18. A nucleic acid encoding the recombinant xylose transporter protein of claim 1.

19. A nucleic acid encoding the recombinant galactose-arabinose transporter protein of claim 9.

20. A method of transporting xylose into a yeast cell, said method comprising: (i) contacting a yeast cell comprising the recombinant xylose transporter protein of claim 1 with a xylose compound; and

(ii) allowing said recombinant xylose transporter protein to transport said xylose compound into said yeast cell.

21. The method of claim 20, wherein said xylose compound forms part of lignocellulosic biomass, hemicellulose, or xylan.

22. The method of claim 20, wherein said yeast cell metabolizes said xylose compound.

23. The method of claim 20, wherein said yeast cell converts said xylose compound to a biofuel.

24. The method of claim 20, wherein said recombinant xylose transporter protein has a binding affinity of at least 0.7 mM for said xylose compound.

25. The method of claim 20, wherein said recombinant xylose transporter protein has a rate of at least 15 nmol min"1 gDCW"1 of transporting said xylose compound into said yeast cell.

26. A method of transporting galactose or arabinose into a yeast cell, said method comprising:

(i) contacting a yeast cell comprising the recombinant galactose-arabinose transporter protein of claim 9 with a galactose compound or an arabinose compound; and

(ii) allowing said recombinant galactose-arabinose transporter protein to transport said galactose compound or said arabinose compound into said yeast cell.

27. The method of claim 26, wherein said recombinant galactose-arabinose transporter protein is contacted with an arabinose compound.

28. The method of claim 27, wherein said arabinose compound forms part of lignocellulosic biomass, hemicellulose or arabinoxylan.

29. The method of claim 26, wherein said yeast cell metabolizes said arabinose compound.

30. The method of claim 26, wherein said yeast cell converts said arabinose compound to a biofuel.

31. The method of claim 26, wherein said recombinant galactose-arabinose transporter protein is contacted with a galactose compound.

32. The method of claim 31, wherein said galactose compound forms a part of lignocellulosic biomass, hemicellulose, or galactan.

33. The method of claim 31 , wherein said yeast cell metabolizes said galactose compound.

34. The method of claim 31 , wherein said yeast cell converts said galactose compound to a biofuel.

35. The method of claim 20, wherein said yeast cell is a S. stipitis yeast cell, a C. intermedia yeast cell, a 5*. cerevisiae yeast cell, a D. hansenii yeast cell, or a 7. lipolytica yeast cell.

36. The method of claim 26, wherein said yeast cell is a 5. stipitis yeast cell, a C. intermedia yeast cell, a 5*. cerevisiae yeast cell, a D. hansenii yeast cell, or a 7. lipolytica yeast cell.