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1. WO2015069742 - TISSUE ARRAY FOR CELL SPHEROIDS AND METHODS OF USE

Publication Number WO/2015/069742
Publication Date 14.05.2015
International Application No. PCT/US2014/064091
International Filing Date 05.11.2014
IPC
C12N 5/07 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
C40B 40/02 2006.1
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
CPC
C12N 2503/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2503Use of cells in diagnostics
C12N 2533/76
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2533Supports or coatings for cell culture, characterised by material
70Polysaccharides
76Agarose, agar-agar
C12N 5/0062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0062General methods for three-dimensional culture
C12N 5/0667
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0662Stem cells
0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
G01N 1/30
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Applicants
  • THE JOHNS HOPKINS UNIVERSITY [US]/[US]
Inventors
  • BEACHLEY, Vince
  • ELISSEEFF, Jennifer, H.
Agents
  • DOERING, Andrea
Priority Data
61/900,09005.11.2013US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TISSUE ARRAY FOR CELL SPHEROIDS AND METHODS OF USE
(FR) RÉSEAU TISSULAIRE POUR SPHÉROÏDES CELLULAIRES ET PROCÉDÉS D'UTILISATION
Abstract
(EN) The present invention generally features methods of preparing a microarray of cell spheroids, methods of preparing a micromold for embedding spheroids for histology, and methods of screening a library of agents.
(FR) La présente invention concerne d'une manière générale des procédés de préparation d'un microréseau de sphéroïdes cellulaires, des procédés de préparation d'une micromoule pour incorporer des sphéroïdes en vue d'une analyse histologique, et des procédés de criblage d'une bibliothèque d'agents.
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