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1. (WO2015048725) CULTURE HARVESTING IN AUTOMATED BIOREACTOR SYSTEM

Pub. No.:    WO/2015/048725    International Application No.:    PCT/US2014/058258
Publication Date: Fri Apr 03 01:59:59 CEST 2015 International Filing Date: Wed Oct 01 01:59:59 CEST 2014
IPC: C12N 5/04
Applicants: WEYERHAEUSER NR COMPANY
Inventors: SWANDA, Anthony, P.
BROWNELL, Patrick, M.
Title: CULTURE HARVESTING IN AUTOMATED BIOREACTOR SYSTEM
Abstract:
A system for harvesting totipotent plant tissue culture can include a sterile enclosure, a plurality of bioreactors in the sterile enclosure, at least one agitator in the sterile enclosure, and a culture harvest system. The at least one agitator can be configured to agitate culture within the plurality of bioreactors. The culture harvest system can include tubing configured to connect to a port of one of the plurality of bioreactors in the sterile enclosure, a culture harvest container, and a pump configured to pump culture from the one of the plurality of bioreactors into the culture harvest container via the tubing.