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1. (WO2015046869) WATER-SOLUBLE EXPRESSION AND PURIFICATION METHOD OF HUMAN GCSF RECOMBINANT PROTEIN HAVING BIOLOGICAL ACTIVITIES
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2015/046869 International Application No.: PCT/KR2014/008876
Publication Date: 02.04.2015 International Filing Date: 24.09.2014
IPC:
C12N 15/27 (2006.01) ,C12N 15/70 (2006.01) ,C07K 14/535 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
19
Interferons; Lymphokines; Cytokines
27
Colony stimulating factors
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
53
Colony-stimulating factor (CSF)
535
Granulocyte CSF; Granulocyte-macrophage CSF
Applicants:
울산대학교 산학협력단 UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION [KR/KR]; 울산시 남구 대학로 93 93 Daehak-ro Nam-gu Ulsan 680-749, KR
Inventors:
최한석 CHOE, Han; KR
유한봉 RYU, Han Bong; KR
도비항 DO, Bich Hang; VN
Agent:
특허법인 태백 TAEBAEK INTELLECTUAL PROPERTY LAW FIRM; 서울시 금천구 가산디지털1로151 이노플렉스1차 601호 #601 Innoplex 1 cha, 151Gasandigital 1-ro Geumcheon-gu Seoul 153-803, KR
Priority Data:
10-2013-011443226.09.2013KR
Title (EN) WATER-SOLUBLE EXPRESSION AND PURIFICATION METHOD OF HUMAN GCSF RECOMBINANT PROTEIN HAVING BIOLOGICAL ACTIVITIES
(FR) PROCÉDÉ D'EXPRESSION ET DE PURIFICATION DES FRACTIONS HYDROSOLUBLES D'UNE PROTÉINE GCSF HUMAINE RECOMBINÉE AYANT DES ACTIVITÉS BIOLOGIQUES
(KO) 생물학적 활성을 가진 인간 GCSF 재조합 단백질의 수용성 발현 및 정제방법
Abstract:
(EN) The present invention relates to a water-soluble expression and purification method of a human GCSF recombinant protein having biological activities using an N-terminal tag, and provides a recombinant expression vector containing a solubility enhancing tag and a human granulocyte colony-stimulating factor (hGCSF) gene. In addition, provided are: a recombinant microorganism transformed with the recombinant expression vector; a method for producing an hGCSF recombinant protein (rhGCSF); and a rhGCSF produced by the production method.
(FR) Cette invention concerne un procédé d'expression et de purification des fractions hydrosolubles d'une protéine GCSF humaine recombinée ayant des activités biologiques faisant appel à une étiquette N-terminale, et un vecteur d'expression recombiné contenant une étiquette améliorant la solubilité et un gène du facteur de stimulation des colonies granulocytaires humaines (hGCSF). De plus, un micro-organisme recombiné transformé à l'aide du vecteur d'expression recombiné ; un procédé de production d'une protéine hGCSF recombinée (rhGCSF) ; et une rhGCSF obtenue par le procédé de production selon l'invention sont en outre décrits.
(KO) 본 발명은 N-말단 태그를 이용한 생물학적 활성을 가진 인간 GCSF 재조합 단백질의 수용성 발현 및 정제방법에 관한 것으로서, 수용성 향상 태그(solubility enhancing tag) 및 인간 과립구 생성 촉진인자(human granulocyte colony-stimulating factor; hGCSF) 유전자를 포함하는 재조합 발현벡터를 제공한다. 또한 상기 재조합 발현벡터로 형질전환된 재조합 미생물을 제공하며, 상기 hGCSF 재조합 단백질(rhGCSF)의 생산방법 및 상기 생산방법에 의해 생산된 hGCSF 재조합 단백질(rhGCSF)을 제공한다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)