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1. (WO2015045741) BUFFER COMPOSITION FOR HYBRIDIZATION USE, AND HYBRIDIZATION METHOD

Pub. No.:    WO/2015/045741    International Application No.:    PCT/JP2014/072865
Publication Date: Fri Apr 03 01:59:59 CEST 2015 International Filing Date: Tue Sep 02 01:59:59 CEST 2014
IPC: C12Q 1/68
C12N 15/09
G01N 33/53
G01N 37/00
Applicants: TOYO KOHAN CO., LTD.
東洋鋼鈑株式会社
YAMAGUCHI UNIVERSITY
国立大学法人山口大学
Inventors: KAMEI Shuichi
亀井 修一
HOSOYA Mayuko
細谷 真悠子
OKA Masaaki
岡 正朗
HAZAMA Shoichi
硲 彰一
TSUNEDOMI Ryouichi
恒富 亮一
Title: BUFFER COMPOSITION FOR HYBRIDIZATION USE, AND HYBRIDIZATION METHOD
Abstract:
The purpose of the present invention is to prevent the occurrence of non-specific hybridization with a nucleic acid probe to achieve an excellent efficiency of detection of a target nucleic acid even in the presence of a non-target nucleic acid. A buffer composition which can be used for the hybridization of a target nucleic acid containing a nucleotide base to be detected with a nucleic acid probe containing a nucleotide sequence complementary to the target nucleic acid, and which comprises a blocking nucleic acid that contains a nucleotide sequence complementary to a non-target nucleic acid, wherein the non-target nucleic acid contains a nucleotide base that is not to be detected and corresponds to the nucleotide base to be detected.