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|1. (WO2015045741) BUFFER COMPOSITION FOR HYBRIDIZATION USE, AND HYBRIDIZATION METHOD|
|Applicants:||TOYO KOHAN CO., LTD.
|Title:||BUFFER COMPOSITION FOR HYBRIDIZATION USE, AND HYBRIDIZATION METHOD|
The purpose of the present invention is to prevent the occurrence of non-specific hybridization with a nucleic acid probe to achieve an excellent efficiency of detection of a target nucleic acid even in the presence of a non-target nucleic acid. A buffer composition which can be used for the hybridization of a target nucleic acid containing a nucleotide base to be detected with a nucleic acid probe containing a nucleotide sequence complementary to the target nucleic acid, and which comprises a blocking nucleic acid that contains a nucleotide sequence complementary to a non-target nucleic acid, wherein the non-target nucleic acid contains a nucleotide base that is not to be detected and corresponds to the nucleotide base to be detected.