Search International and National Patent Collections

1. (WO2015045593) METHOD AND KIT FOR ANALYZING TARGET

Pub. No.:    WO/2015/045593    International Application No.:    PCT/JP2014/069390
Publication Date: Fri Apr 03 01:59:59 CEST 2015 International Filing Date: Thu Jul 24 01:59:59 CEST 2014
IPC: C12Q 1/34
C12N 15/09
C12Q 1/68
Applicants: NEC SOLUTION INNOVATORS, LTD.
NECソリューションイノベータ株式会社
Inventors: YOSHIDA Yoshihito
吉田 嘉仁
HORII Katsunori
堀井 克紀
WAGA Iwao
和賀 巌
Title: METHOD AND KIT FOR ANALYZING TARGET
Abstract:
Provided are: a novel method for analyzing a target easily using a binding nucleic acid molecule; and an analysis kit for use in the method. The analysis method according to the present invention comprises: a complex formation step of bringing a binding nucleic acid molecule capable of binding to a target into contact with a sample to form a complex of the binding nucleic acid molecule and the target contained in the sample; a nuclease treatment step of liberating a nucleic acid monomer from a fraction of the complex and/or a fraction that does not contain the complex by the treatment with a nuclease; an enzymatic treatment step of reacting the liberated nucleic acid monomer with an enzyme for which the substrate is a nucleic acid monomer; a detection step of detecting the enzymatic reaction; and an analysis step of analyzing the target that forms the complex from the results of the detection of the enzymatic reaction.