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1. (WO2015044456) IMPROVED BIOSENSOR-BASED DETECTION SYSTEM
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2015/044456 International Application No.: PCT/EP2014/070954
Publication Date: 02.04.2015 International Filing Date: 30.09.2014
IPC:
C12Q 1/25 (2006.01) ,C12Q 1/68 (2006.01) ,C12N 15/11 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
25
involving enzymes not classifiable in groups C12Q1/26-C12Q1/7097
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
Applicants:
DANMARKS TEKNISKE UNIVERSITET [DK/DK]; Anker Engelunds Vej 101 A DK-2800 Kgs. Lyngby, DK
Inventors:
RUGBJERG, Peter; DK
GENEE, Hans Jasper; DK
SOMMER, Morten; DK
Agent:
LÖVQVIST, Anna; DK
Priority Data:
13186673.330.09.2013EP
Title (EN) IMPROVED BIOSENSOR-BASED DETECTION SYSTEM
(FR) SYSTÈME DE DÉTECTION À BASE DE BIOCAPTEUR AMÉLIORÉ
Abstract:
(EN) Described is a new biosensor-based detection system for effector compounds, useful for in vivo applications in e.g. screening and selecting of cells which produce a small molecule effector compound or which take up a small molecule effector compound from its environment. The detection system comprises a protein or RNA-based biosensor for the effector compound which indirectly regulates the expression of a reporter gene via two hybrid proteins, providing for fewer false signals or less 'noise', tuning of sensitivity or other advantages over conventional systems where the biosensor directly regulates the reporter gene.
(FR) La présente invention porte sur un nouveau système de détection à base de biocapteur pour des composés effecteurs, utile pour des applications in vivo dans, par exemple, le criblage et la sélection de cellules qui produisent un composé effecteur à petite molécule ou qui absorbent un composé effecteur à petite molécule à partir de leur environnement. Le système de détection comprend un biocapteur à base de protéine ou d'ARN pour le composé effecteur qui régule indirectement l'expression d'un gène rapporteur par l'intermédiaire de deux protéines hybrides, ce qui permet d'obtenir moins de faux signaux ou moins de bruit, réglage la sensibilité et d'autres avantages par rapport à des systèmes classiques où le biocapteur régule directement le gène rapporteur.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)