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1. (WO2015044206) STABILIZED INHIBITOR PROTEASE VARIANTS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2015/044206 International Application No.: PCT/EP2014/070377
Publication Date: 02.04.2015 International Filing Date: 24.09.2014
IPC:
C12N 9/54 (2006.01) ,C11D 3/386 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
54
bacteria being Bacillus
C CHEMISTRY; METALLURGY
11
ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
D
DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
3
Other compounding ingredients of detergent compositions covered in group C11D1/101
16
Organic compounds
38
Products with no well-defined composition
386
Preparations containing enzymes
Applicants:
HENKEL AG & CO. KGAA [DE/DE]; Henkelstr. 67 40589 Düsseldorf, DE
Inventors:
HELLMUTH, Hendrik; DE
LAUFS, Brian; DE
AYDEMIR, Ayhan; DE
WEBER, Thomas; DE
O'CONNELL, Timothy; DE
JAKOB, Felix; DE
MARTINEZ, Ronny Ernesto; DE
SCHWANEBERG, Ulrich; BE
BEZDEK, Siegfried Andreas; DE
Priority Data:
10 2013 219 467.626.09.2013DE
Title (EN) STABILIZED INHIBITOR PROTEASE VARIANTS
(FR) VARIANTES DE PROTÉASES INHIBITRICES STABILISÉES
(DE) STABILISIERTE INHIBITOR-PROTEASEVARIANTEN
Abstract:
(EN) The invention relates to proteases comprising an amino acid sequence which has at least 70% of the sequential identity of the amino acid sequence specified in SEQ ID NO. 1 over the entire amino acid sequence length, which has the amino acid groups 160G and 185R, and in which the amino acids in at least one, preferably two, preferably three, in particular four, particularly preferably five, and most preferably six of the positions that correspond to the positions 39, 74, 253, and 3, 4, 199 according to SEQ ID NO. 1 are substituted with 39E, 74D, 253D, 3T, 4I, and 199I. The invention also relates to the production and use of said proteases. Such proteases exhibit a very good stability, in particular a very good surfactant stability, while simultaneously exhibiting a good inhibitor performance, in particular with benzylmalonic acid.
(FR) L'invention concerne des protéases comprenant une séquence d'acides aminés qui présente sur toute sa longueur une identité de séquence d'au moins 70% par rapport à la séquence d'acides aminés indiquée dans la SEQ ID N° 1, qui contient les résidus acides aminés 160G et 185R et dans laquelle les acides aminés sont remplacés par 39E, 74D, 253D, 3T, 4I et 199I dans au moins une, de préférence 2, de préférence 3, en particulier 4, de façon particulièrement préférée 5 et de façon tout particulièrement préférée 6 des positions correspondant aux positions 39, 74, 253 ainsi que 3, 4, 199 de la SEQ ID N° 1. L'invention concerne en outre leur préparation et leur utilisation. Ces protéases possèdent une très bonne stabilité, en particulier aux surfactants, en même temps que de bonnes performances d'inhibition, en particulier avec l'acide benzylmalonique.
(DE) Die Erfindung betrifft Proteasen umfassend eine Aminosäuresequenz, die mindestens 70% Sequenzidentität zu der in SEQ ID NO. 1 angegebenen Aminosäuresequenz über deren Gesamtlänge aufweist, die die Aminosäurereste 160G und 185R aufweist und in der die Aminosäuren in mindestens einer, vorzugsweise 2, bevorzugt 3, insbesondere 4, besonders bevorzugt 5, ganz besonders bevorzugt sechs der Positionen, die den Positionen 39, 74, 253 sowie 3, 4, 199 gemäß SEQ ID NO. 1 entsprechen, substituiert sind zu 39E, 74D, 253D, 3T, 4I und 199I sowie deren Herstellung und Verwendung. Derartige Proteasen zeigen eine sehr gute Stabilität, insbesondere Tensidstabilität, bei gleichzeitig guter Inhibitorleistung, insbesondere mit Benzylmalonsäure.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)