Search International and National Patent Collections

1. (WO2015043160) METHOD FOR PROMOTING FORMATION OF HEPATITIS B SURFACE ANTIGEN COVALENTLY CROSSLINKED PARTICLES EXPRESSED BY SACCHAROMYCES CEREVISIAE

Pub. No.:    WO/2015/043160    International Application No.:    PCT/CN2014/075311
Publication Date: Fri Apr 03 01:59:59 CEST 2015 International Filing Date: Tue Apr 15 01:59:59 CEST 2014
IPC: C07K 14/02
A61K 39/29
A61P 31/12
Applicants: SHENZHEN KANGTAI BIOLOGICAL PRODUCTS CO., LTD.
深圳康泰生物制品股份有限公司
Inventors: ZHU, Zhengyu
朱征宇
RAO, Hongchong
饶洪冲
DUAN, Yan
段琰
Title: METHOD FOR PROMOTING FORMATION OF HEPATITIS B SURFACE ANTIGEN COVALENTLY CROSSLINKED PARTICLES EXPRESSED BY SACCHAROMYCES CEREVISIAE
Abstract:
Provided in the present invention is a method for promoting the formation of hepatitis B surface antigen covalently crosslinked particles expressed by Saccharomyces cerevisiae, comprising the following steps: maintaining recombinant hepatitis B surface antigens expressed by Saccharomyces cerevisiae under the condition of a temperature of 4-100°C for 3 months to 5 minutes, to make polypeptides, which are non-covalently bound to each other, inter-chains and dimers formed by single polypeptides convert to disulfide bonds between polypeptides, and forming covalently crosslinked antigen particles. The present invention can promote the formation of high immunity antigen particles without the addition of chemicals.