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1. (WO2015042649) A QUANTITATIVE ASSAY FOR TARGET DNA IN A MIXED SAMPLE COMPRISING TARGET AND NON-TARGET DNA

Pub. No.:    WO/2015/042649    International Application No.:    PCT/AU2014/000942
Publication Date: Fri Apr 03 01:59:59 CEST 2015 International Filing Date: Sat Sep 27 01:59:59 CEST 2014
IPC: C12Q 1/68
Applicants: MURDOCH CHILDREN'S RESEARCH INSTITUTE
Inventors: SLATER, Howard Robert
BRUNO, Damien Luis
GANESAMOORTHY, Devika
Title: A QUANTITATIVE ASSAY FOR TARGET DNA IN A MIXED SAMPLE COMPRISING TARGET AND NON-TARGET DNA
Abstract:
The present disclosure relates generally to an assay to detect and quantitate target nucleic acid in a mixture of target and non-target nucleic acid, kits useful for same and its use in non-invasive diagnostic methodologies. The assay comprises identifying copy number variation (CNV) polymorphisms present in the target nucleic acid and absent in non-target nucleic acid. Embodiments disclosed include quantitating the level of fetal DNA in sample of maternal DNA and fetal DNA, and quantitating the level of donor-derived DNA in sample from a transplant recipient comprising self DNA and non-self or donor DNA.