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1. (WO2015028811) METHODS FOR DETECTING PROTEINS OR BINDING PARTNERS FOR PROTEINS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2015/028811    International Application No.:    PCT/GB2014/052620
Publication Date: 05.03.2015 International Filing Date: 29.08.2014
IPC:
C12Q 1/00 (2006.01), B01L 3/00 (2006.01), G01N 33/543 (2006.01), G01N 33/553 (2006.01), G01N 33/68 (2006.01), G01N 33/72 (2006.01), C12Q 1/28 (2006.01), G01N 27/00 (2006.01)
Applicants: THE UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW [GB/GB]; University Avenue Glasgow Strathclyde G12 8QQ (GB)
Inventors: COOPER, Jonathan; (GB).
REBOUD, Julien; (GB)
Agent: WILLS, Jonathan; (GB)
Priority Data:
1315532.0 30.08.2013 GB
Title (EN) METHODS FOR DETECTING PROTEINS OR BINDING PARTNERS FOR PROTEINS
(FR) PROCÉDÉS DE DÉTECTION DE PROTÉINES
Abstract: front page image
(EN)Provided is a method for the detection of a protein, such as haemoglobin or a metalloprotein, in a test sample, such as faecal sample, the method comprising the steps of: (i) exposing the sample to a surface having a binding moiety and permitting the binding moiety to specifically immobilise the protein in the sample; (ii) permitting the immobilised protein to catalyse a reaction of a first reagent with a second reagent; and (iii) detecting, such as electrochemically detecting, the reaction of the first reagent with the second reagent. The binding moiety may be provided on the surface of an electrode. Also provided is a method for detecting an analyte in a sample, which analyte is a binding partner for a protein, the method comprising the steps of: (i) providing a protein that is immobilised, such as specifically immobilised, to a surface; (ii) exposing the immobilised protein to the analyte in the sample, thereby to bind the analyte to the immobilised protein; (iii) exposing the immobilised protein to a first reagent and a second reagent; and (iv) detecting, such as electrochemically detecting, the reaction of first reagent with the second reagent, wherein the immobilised protein is a catalyst for the reaction of the first reagent with the second reagent in the absence of the analyte, and the binding of the analyte to the immobilised protein alters the catalytic activity of the protein for the reaction of the first reagent with the second reagent. The binding moiety can be an antibody. The first reagent can be tetramethylbenzidine and the second reagent can be hydrogen peroxide.
(FR)Cette invention concerne un procédé pour la détection d'une protéine, telle qu'Hb, dans un échantillon d'essai, le procédé comprenant les étapes consistant : (i) à exposer l'échantillon à une surface portant un fragment de liaison et à laisser le fragment de liaison immobiliser spécifiquement la protéine dans l'échantillon : (ii) à laisser la protéine immobilisée catalyser une réaction d'un premier réactif avec un second réactif; et (iii) à détecter, par exemple par un moyen électrochimique, la réaction du premier réactif avec le second réactif. Le fragment de liaison peut être fourni sur la surface d'une électrode.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)