Processing

Please wait...

Settings

Settings

Goto Application

1. WO2014209145 - THE METHOD FOR DETERMINATION OF PHYLOGENETIC RELATIONS AMONG STAPHYLOCOCCUS SPECIES STRAINS

Publication Number WO/2014/209145
Publication Date 31.12.2014
International Application No. PCT/PL2014/050038
International Filing Date 25.06.2014
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/683
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6827for detection of mutation or polymorphism
683involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
C12Q 1/6888
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
Applicants
  • UNIWERSYTET JAGIELLOŃSKI
Inventors
  • BUKOWSKI, Michał
  • POLAKOWSKA, Klaudia
  • HELBIN, Weronika
  • SITARSKA, Agnieszka
  • NYTKO, Kinga
  • WŁADYKA, Benedykt
Agents
  • BRANDPAT PATENT AND TRADEMARK ATTORNEYS
Priority Data
P.40449728.06.2013PL
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) THE METHOD FOR DETERMINATION OF PHYLOGENETIC RELATIONS AMONG STAPHYLOCOCCUS SPECIES STRAINS
(FR) PROCÉDÉ PERMETTANT DE DÉTERMINER LES RELATIONS PHYLOGÉNÉTIQUES AU SEIN DES SOUCHES DE L'ESPÈCE STAPHYLOCOCCUS
Abstract
(EN)
The new method for species determination and intra-species diversity analysis of Staphylococcus strains was revealed. An exemplary way to achieve this is the use of the polymerase chain reaction (PCR) to obtain the product on the conserved locus template, which is subsequently digested with restriction enzymes (method known as restriction fragments length polymorphism, RFLP). This allows to obtain restriction patterns allowing for species determination. Intra-species diversity analysis for staphylococci is also possible when the RFLP is carried out for properly selected enzymes.
(FR)
Cette invention concerne un nouveau procédé de détermination de l'espèce et d'analyse de la diversité intra-espèces chez Staphylococcus. Une façon d'y parvenir consiste à utiliser la réaction en chaîne de la polymérase (PCR) pour obtenir le produit sur la matrice des locus conservés, et à le soumettre ensuite à digestion par des enzymes de restriction (procédé connu sous le nom de polymorphisme de longueur des fragments de restriction, ou RFLP). Ceci permet d'obtenir des profils de restriction permettant de déterminer les espèces. L'analyse des diversités intra-espèces pour les staphylocoques est également possible quand le RFLP est mis en œuvre pour des enzymes dûment sélectionnées.
Latest bibliographic data on file with the International Bureau