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1. (WO2014208100) METHOD FOR PRODUCING MEGAKARYOCYTES, PLATELETS AND/OR THROMBOPOIETIN USING MESENCHYMAL CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2014/208100 International Application No.: PCT/JP2014/003445
Publication Date: 31.12.2014 International Filing Date: 27.06.2014
IPC:
C12N 5/078 (2010.01) ,C07K 14/47 (2006.01) ,C12N 5/0775 (2010.01) ,C12P 21/02 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
Applicants:
学校法人慶應義塾 KEIO UNIVERSITY [JP/JP]; 東京都港区三田二丁目15番45号 15-45, Mita 2-chome, Minato-ku, Tokyo 1088345, JP
公益財団法人神奈川科学技術アカデミー KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY [JP/JP]; 神奈川県川崎市高津区坂戸3丁目2番1号 2-1, Sakado 3-chome, Takatsu-ku, Kawasaki-shi, Kanagawa 2130012, JP
Inventors:
松原 由美子 MATSUBARA, Yumiko; JP
座間 猛 ZAMA, Takeru; JP
池田 康夫 IKEDA, Yasuo; JP
宇留賀 友佳子 URUGA, Yukako; JP
須田 年生 SUDA, Toshio; JP
松岡 佐保子 MATSUOKA, Sahoko; JP
Agent:
廣田 雅紀 HIROTA, Masanori; JP
Priority Data:
2013-13700428.06.2013JP
2013-13703328.06.2013JP
2014-07005828.03.2014JP
Title (EN) METHOD FOR PRODUCING MEGAKARYOCYTES, PLATELETS AND/OR THROMBOPOIETIN USING MESENCHYMAL CELLS
(FR) PROCÉDÉ DE PRODUCTION DE MÉGACARYOCYTES, DE PLAQUETTES ET/OU DE THROMBOPOÏÉTINE UTILISANT DES CELLULES MÉSENCHYMATEUSES
(JA) 間葉系細胞を利用した巨核球、血小板及び/又はトロンボポエチンの製造方法
Abstract:
(EN) Provided are: a method for producing megakaryocytes and/or platelets, with which it is possible to produce megakaryocytes and/or platelets in vitro from mesenchymal cells such as preadipocytes in a relatively short period of time, easily and in large quantities, and at lower cost and with greater efficiency; and a method for producing TPO easily and in large quantities. A first aspect of this invention is a method for producing megakaryocytes and/or platelets by culturing mesenchymal cells in a basal medium for mesenchymal cell culture that includes iron ions and an iron transporter, and collecting megakaryocytes and/or platelets from the culture. A second aspect of this invention is a method for producing thrombopoietin by culturing mesenchymal cells or megakaryocytes derived from mesenchymal cells in a basal medium for mesenchymal cell culture that includes iron ions and an iron transporter, and collecting thrombopoietin from the culture. A third aspect of this invention is a method for producing thrombopoietin by culturing preadipocytes in a basal medium for preadipocyte culture that includes dexamethasone, 3-isobutyl-1-methylxanthine and insulin, and collecting thrombopoietin from the culture.
(FR) La présente invention concerne : un procédé de production de mégacaryocytes et/ou de plaquettes qui permet de produire des mégacaryocytes et/ou des plaquettes in vitro à partir de cellules mésenchymateuses telles que des préadipocytes, sur une période de temps relativement courte, de manière simple et en grandes quantités, à coût inférieur et avec une efficacité supérieure ; et un procédé permettant de produire de la TPO de manière simple et en grandes quantités. La présente invention concerne selon un premier aspect un procédé permettant de produire des mégacaryocytes et/ou des plaquettes en cultivant des cellules mésenchymateuses dans un milieu basal de culture de cellules mésenchymateuses comprenant des ions de fer ainsi qu'un transporteur de fer, et en récoltant les mégacaryocytes et/ou les plaquettes de la culture. La présente invention concerne selon un deuxième aspect un procédé permettant de produire de la thrombopoïétine en cultivant des cellules mésenchymateuses ou des mégacaryocytes dérivés de cellules mésenchymateuses dans un milieu basal de culture de cellules mésenchymateuses comprenant des ions de fer ainsi qu'un transporteur de fer, et en récoltant la thrombopoïétine de la culture. La présente invention concerne selon un troisième aspect un procédé permettant de produire de la thrombopoïétine en cultivant des préadipocytes dans un milieu basal de culture de préadipocytes comprenant de la dexaméthasone, du 3-isobutyle-1-méthylxanthine et de l'insuline, et en récoltant la thrombopoïétine de la culture.
(JA) 脂肪前駆細胞等の間葉系細胞から巨核球及び/又は血小板を比較的短期間で簡便かつ多量に、しかもより低コスト或いはより効率的に生体外で製造し得る、巨核球及び/又は血小板の製造方法、および、TPOを簡便かつ多量に製造する方法を提供する。第1の本発明は、間葉系細胞を鉄イオン及び鉄輸送体を含む間葉系細胞培養用基本培地で培養し、培養物から巨核球及び/又は血小板を採取する、巨核球及び/又は血小板の製造方法である。第2の本発明は、間葉系細胞又は間葉系細胞由来の巨核球を鉄イオン及び鉄輸送体を含む間葉系細胞培養用基本培地で培養し、培養物からトロンボポエチンを採取する、トロンボポエチンの製造方法である。第3の本発明は、脂肪前駆細胞を、デキサメタゾン、3-イソブチル-1-メチルキサンチン及びインスリンを含む脂肪前駆細胞培養用基本培地で培養し、培養物からトロンボポエチンを採取する、トロンボポエチンの製造方法である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)