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1. (WO2014206376) METHOD OF CLASSIFYING AND COUNTING WHITE BLOOD CELLS ON BASIS OF MORPHOLOGY
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2014/206376 International Application No.: PCT/CN2014/082868
Publication Date: 31.12.2014 International Filing Date: 24.07.2014
IPC:
C12Q 1/04 (2006.01) ,G01N 33/49 (2006.01) ,C12R 1/91 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
487
of liquid biological material
49
blood
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
91
Cell lines
Applicants: SUZHOU CHUANGJI BIOLOGICAL TECHNOLOGY CO., LTD[CN/CN]; Room 303, Building C2, No.218, Xing Hu Rd., Suzhou Industrial Park Suzhou, Jiangsu 215123, CN
Inventors: YIN, Yuefeng; CN
Agent: NANJING ZHENGLIAN INTELLECTUAL PROPERTY AGENCY CO., LTD; 27/F., Blog.A, No.1 Hanzhongmen Rd. Nanjing, Jiangsu 210029, CN
Priority Data:
201310254872.625.06.2013CN
Title (EN) METHOD OF CLASSIFYING AND COUNTING WHITE BLOOD CELLS ON BASIS OF MORPHOLOGY
(FR) PROCÉDÉ DE CLASSIFICATION ET DE COMPTAGE DE GLOBULES BLANCS SUR LA BASE DE LA MORPHOLOGIE
(ZH) 一种基于形态学分类计数白细胞的方法
Abstract:
(EN) A method of classifying and counting white blood cells on the basis of morphology is disclosed: Wright's staining a peripheral blood smear, then obtaining an image by means of a microscope; processing the image by means of a computer system, performing morphology parameter analysis on white blood cells, and differentiating said cells into four areas, i.e. a lymphocyte area, a monocyte area, a granulocyte area and a cell extinction area; performing morphology parameter analysis on the cells differentiated into said areas in step 1 to further differentiate said cells into monocytes, lymphocytes, eosinophil granulocytes and neutrophil granulocytes; and creating statistics on classification and count of differentiated white blood cell types. Use of a computer system to analyze and process an image, and utilization of several simple morphology parameters to generate a two-dimensional scatter plot, so as to represent the cells in the form of a statistical graph, allow for the accurate and rapid classification of white blood cells and for the automated counting of said cells. Compared to manual counting, the steps of the present invention are simple and quick and markedly increase the speed and accuracy of diagnosis.
(FR) L'invention concerne un procédé de classification et de comptage de globules blancs sur la base de la morphologie et comprend les étapes suivantes : effectuer une coloration de Wright d'un frottis de sang périphérique, puis obtenir une image au moyen d'un microscope, traiter l'image au moyen d'un système informatique, effectuer une analyse des paramètres de morphologie sur des globules blancs et différencier lesdites cellules en quatre zones, c.-à-d. une zone de lymphocytes, une zone de monocytes, une zone de granulocytes et une zone d'extinction de cellules, effectuer une analyse des paramètres de morphologie sur les cellules différenciées dans lesdites zones à l'étape 1, de manière à mieux différencier encore lesdites cellules, en monocytes, lymphocytes, granulocytes éosinophiles et granulocytes neutrophiles, et à créer des statistiques sur la classification et le comptage des types de globules blancs différenciés. L'utilisation d'un système informatique pour analyser et traiter une image ainsi que l'utilisation de plusieurs paramètres de morphologie simple, pour générer un diagramme de dispersion bidimensionnel, de manière à représenter les cellules sous la forme d'un graphique statistique, permet la classification rapide et précise de globules blancs et le comptage automatique desdites cellules. Comparativement au comptage manuel, les étapes de la présente invention sont simples et rapides à mettre en œuvre et augmentent considérablement la vitesse et la précision du diagnostic.
(ZH) 本发明公开了一种基于形态学分类计数白细胞的方法:外周血涂片经瑞氏染色后,利用显微镜采集图像;图像经计算机系统处理后对白细胞进行形态学参数分析,将白细胞划分为淋巴细胞区、单核细胞区、粒细胞区及消亡细胞区四个区域;将前一步骤所划分出的区域细胞进行形态学参数分析,将细胞进一步精确区分出单核细胞、淋巴细胞、嗜酸粒细胞和中性粒细胞;将区分出来的白细胞种类进行分类计数统计。经计算机系统分析处理图像,运用几种简单的形态学参数作出二维散点图,将所有细胞以统计图的方式呈现,实现了白细胞快速精准分类,白细胞计数自动化,与人工计数相比,步骤简单快速,诊断速度与准确性大幅提高。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)