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|1. (WO2014206210) METHOD FOR PREPARING EPTIFIBATIDE|
|Applicants:||HYBIO PHARMACEUTICAL CO., LTD.
|Title:||METHOD FOR PREPARING EPTIFIBATIDE|
Provided is a method for preparing eptifibatide, wherein the method comprises: obtaining an eptifibatide refined peptide solution; and obtaining and freeze-drying an eptifibatide refined peptide concentrate after rotary evaporation of the eptifibatide refined peptide solution, wherein the concentration of the eptifibatide refined peptide concentrate is 15-30 mg/ml and the temperature of rotary evaporation is 251°C-35°C. The preparation method of the eptifibatide refined peptide solution is as follows: coupling Cys with a resin to obtain a Cys-resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by gradually coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpr; and obtaining the eptifibatide refined peptide solution through oxidation, cleavage, purification and transfer to salt.