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1. (WO2014206210) METHOD FOR PREPARING EPTIFIBATIDE

Pub. No.:    WO/2014/206210    International Application No.:    PCT/CN2014/079935
Publication Date: Thu Jan 01 00:59:59 CET 2015 International Filing Date: Tue Jun 17 01:59:59 CEST 2014
IPC: C07K 7/06
C07K 1/06
C07K 1/02
C07K 1/04
Applicants: HYBIO PHARMACEUTICAL CO., LTD.
深圳翰宇药业股份有限公司
Inventors: KANG, Xu
康旭
LIU, Jian
刘建
MA, Yaping
马亚平
YUAN, Jiancheng
袁建成
Title: METHOD FOR PREPARING EPTIFIBATIDE
Abstract:
Provided is a method for preparing eptifibatide, wherein the method comprises: obtaining an eptifibatide refined peptide solution; and obtaining and freeze-drying an eptifibatide refined peptide concentrate after rotary evaporation of the eptifibatide refined peptide solution, wherein the concentration of the eptifibatide refined peptide concentrate is 15-30 mg/ml and the temperature of rotary evaporation is 251°C-35°C. The preparation method of the eptifibatide refined peptide solution is as follows: coupling Cys with a resin to obtain a Cys-resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by gradually coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpr; and obtaining the eptifibatide refined peptide solution through oxidation, cleavage, purification and transfer to salt.