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1. (WO2014204050) AXENIC INOCULATION SYSTEM FOR MICROALGAE USING TR TUBE OF TRIIODIDE RESIN AND METHOD FOR CULTURING AXENIC CULTURE

Pub. No.:    WO/2014/204050    International Application No.:    PCT/KR2013/008012
Publication Date: Thu Dec 25 00:59:59 CET 2014 International Filing Date: Fri Sep 06 01:59:59 CEST 2013
IPC: C12M 3/06
C12M 1/24
C12N 1/12
Applicants: ADVANCED BIOMASS R&D CENTER
재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단
Inventors: KWON, Jong Hee
권종희
YANG, Ji Won
양지원
JUNG, Ju Young
정주영
NAM, Gi Bok
남기복
PARK, Min Sung
박민성
KANG, Nam Kyu
강남규
SUNG, Min Gyu
성민규
Title: AXENIC INOCULATION SYSTEM FOR MICROALGAE USING TR TUBE OF TRIIODIDE RESIN AND METHOD FOR CULTURING AXENIC CULTURE
Abstract:
A triiodide resin (TR) tube of the present invention has a cylindrical shape. A resin exhaust preventing part is provided at a lower portion of the TR tube, and a triiodide resin is received inside the TR tube. A contaminated culture passes through the TR tube to allow an axenic culture to flow out. When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. An axenic inoculation system for microalgae using the TR tube of the present invention comprises the steps of: sterilizing a photobioreactor (PBR) with peracetic acid, washing the PBR with purified water, and filling the PBR with a sterilized culture; allowing a microalgae culture contaminated with bacteria to pass through a TR tube attached on an upper portion of the PBR, thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture microalgae. According to the present invention, the microalgae contaminated with bacteria are sterilized together with bacteria in external air flowing in the TR tube while passing through the TR tube, thereby causing no secondary contamination at the time of inoculation or culture.