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1. (WO2014201986) MONOCLONAL MESENCHYMAL STEM CELL GENERATION METHOD AND USE THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2014/201986 International Application No.: PCT/CN2014/079970
Publication Date: 24.12.2014 International Filing Date: 16.06.2014
IPC:
C12N 5/0775 (2010.01) ,A61K 35/12 (2006.01) ,A61P 7/00 (2006.01) ,A61P 37/00 (2006.01) ,A61P 25/00 (2006.01) ,A61P 3/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
7
Drugs for disorders of the blood or the extracellular fluid
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
37
Drugs for immunological or allergic disorders
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
25
Drugs for disorders of the nervous system
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
3
Drugs for disorders of the metabolism
08
for glucose homeostasis
10
for hyperglycaemia, e.g. antidiabetics
Applicants: ZHANG, Wenwei[CN/CN]; CN
Inventors: ZHANG, Wenwei; CN
Agent: TSINGYIHUA INTELLECTUAL PROPERTY LLC; Room 301, Trade Building, Zhaolanyuan, Tsinghua University, Qinghuayuan, Haidian District Beijing 100084, CN
Priority Data:
201310253266.221.06.2013CN
Title (EN) MONOCLONAL MESENCHYMAL STEM CELL GENERATION METHOD AND USE THEREOF
(FR) PROCÉDÉ DE PRODUCTION DE CELLULE SOUCHE MÉSENCHYMATEUSE MONOCLONALE ET UTILISATION CORRESPONDANTE
(ZH) 建立单克隆间充质干细胞的方法及其应用
Abstract:
(EN) Provided is a mesenchymal stem cell separation method, comprising: A) utilizing collagenase to digest an animal sample, and dispersing cells contained in the animal sample to obtain a cell mixture comprising stem cells; B) introducing the nucleic acid molecule of encoded reporter protein into at least some of the cells of the cell mixture, the nucleic acid molecule of the encoded reporter protein being operably connected to the specificity promoter of the mesenchymal stem cell to express the reporter protein in the mesenchymal stem cell; and C) utilizing FACS to sort and obtain the mesenchymal stem cell expressing the reporting protein, the mesenchymal stem cell being a single cell.
(FR) L'invention concerne un procédé de séparation de cellule souche mésenchymateuse consistant à : A) utiliser une collagénase pour digérer un échantillon animal et disperser les cellules contenues dans l'échantillon animal pour obtenir un mélange de cellules comprenant des cellules souches ; B) introduire la molécule d'acide nucléique de la protéine rapporteuse codée dans au moins certaines des cellules du mélange de cellules, la molécule d'acide nucléique de la protéine rapporteuse codée étant reliée fonctionnellement aux promoteurs de spécificité de la cellule souche mésenchymateuse pour exprimer la protéine rapporteuse dans la cellule souche mésenchymateuse ; et C) utiliser FACS (tri de cellules activées par fluorescence) pour trier et obtenir la cellule souche mésenchymateuse exprimant la protéine rapporteuse, la cellule souche mésenchymateuse étant une cellule unique.
(ZH) 本发明提供一种分离间充质干细胞的方法,包括:A)利用胶原酶对动物样本进行消化,分散所述动物样本中所包含的细胞,以便获得包含干细胞的细胞混合物;B)将编码报告蛋白的核酸分子引入所述细胞混合物的至少一部分细胞中,其中,所述编码报告蛋白的核酸分子与间充质干细胞特异性启动子可操纵地相连,以便在间充质干细胞中表达所述报告蛋白;以及C)利用FACS,分选获得表达所述报告蛋白的间充质干细胞,其中,所述间充质干细胞呈单个细胞的形式。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)