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1. (WO2014201273) HIGH-THROUGHPUT RNA-SEQ

Pub. No.:    WO/2014/201273    International Application No.:    PCT/US2014/042159
Publication Date: Fri Dec 19 00:59:59 CET 2014 International Filing Date: Fri Jun 13 01:59:59 CEST 2014
IPC: C12Q 1/68
Applicants: THE BROAD INSTITUTE, INC.
PRESIDENT AND FELLOWS OF HARVARD COLLEGE
MIKKELSEN, Tarjei
SOUMILLON, Magali
Inventors: MIKKELSEN, Tarjei
SOUMILLON, Magali
Title: HIGH-THROUGHPUT RNA-SEQ
Abstract:
The present invention relates generally to methods for single-cell nucleic acid profiling, and nucleic acids useful in those methods. For example, it concerns using barcode sequences to track individual nucleic acids at single-cell resolution, utilizing template switching and sequencing reactions to generate the nucleic acid profiles. These methods and compositions are also applicable to other starting materials, such as cell and tissue lysates or extracted/purified RNA.