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1. (WO2014197096) ASSAY ENHANCEMENT BY SELECTIVE DEPOSITION AND BINDING ON AMPLIFICATION STRUCTURES

Pub. No.:    WO/2014/197096    International Application No.:    PCT/US2014/029979
Publication Date: Fri Dec 12 00:59:59 CET 2014 International Filing Date: Sun Mar 16 00:59:59 CET 2014
IPC: G01N 21/63
G01N 21/27
Applicants: THE TRUSTEES OF PRINCETON UNIVERSITY
Inventors: CHOU, Stephen Y.
Title: ASSAY ENHANCEMENT BY SELECTIVE DEPOSITION AND BINDING ON AMPLIFICATION STRUCTURES
Abstract:
This disclosure provides, among other things, a method for enhancing detection of an analyte that is bound to a substrate comprising a signal amplification layer on a surface of the substrate, wherein the signal amplification layer comprises high-amplification regions and low-amplification regions, and the high-amplification regions amplify signals at said surface more than the low-amplification regions. The method comprises selectively masking the low-amplification regions of the substrate, thereby increasing the probability that an analyte will bind to a high-amplification region and be detected.