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1. (WO2014196549) METHOD FOR THREE-DIMENSIONAL CULTURE OF CELLS USING FIBER-ON-FIBER AND SUBSTRATE THEREFOR

Pub. No.:    WO/2014/196549    International Application No.:    PCT/JP2014/064789
Publication Date: Fri Dec 12 00:59:59 CET 2014 International Filing Date: Wed Jun 04 01:59:59 CEST 2014
IPC: C12M 3/00
C12N 1/04
C12N 5/0735
C12N 5/10
Applicants: KYOTO UNIVERSITY
国立大学法人京都大学
Inventors: KAMEI, Kenichiro
亀井 謙一郎
NAKATSUJI, Norio
中辻 憲夫
LIU, Li
劉 莉
Title: METHOD FOR THREE-DIMENSIONAL CULTURE OF CELLS USING FIBER-ON-FIBER AND SUBSTRATE THEREFOR
Abstract:
The present invention provides, for example, the following: a substrate for cell culture, which is a support on which are contained nanofibers formed from a biopolymer selected from the group consisting of gelatin, collagen, and cellulose; a cell cryogen containing the substrate; a method for cell culture comprising inoculation of cells onto the substrate and static culture of the cells; the same method comprising dissociation of the cells from the substrate using an enzyme-free dissociation solution, inoculation of the cells onto another of the culture substrate, and further static culture of the cells; in particular, a method comprising separation of subcultured cells into single cells; and a method for cryopreservation of pluripotent stem cells using the cell cryogen.