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1. (WO2014191580) EXTRACELLULAR DITERPENE PRODUCTION

Pub. No.:    WO/2014/191580    International Application No.:    PCT/EP2014/061398
Publication Date: Fri Dec 05 00:59:59 CET 2014 International Filing Date: Tue Jun 03 01:59:59 CEST 2014
IPC: C12P 15/00
C12P 19/56
A23L 1/236
A23L 2/60
C12N 9/90
C12N 9/88
C12N 9/02
C12N 9/10
C12N 15/52
Applicants: DSM IP ASSETS B.V.
Inventors: BOER, Viktor Marius
BROERS, Nicolette, Jasmijn
LAWRENCE, Adam, G.
Title: EXTRACELLULAR DITERPENE PRODUCTION
Abstract:
The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.