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1. (WO2014191383) METHOD FOR LASER MICRODISSECTION AND LASER MICRODISSECTION SYSTEM

Pub. No.:    WO/2014/191383    International Application No.:    PCT/EP2014/060899
Publication Date: Fri Dec 05 00:59:59 CET 2014 International Filing Date: Wed May 28 01:59:59 CEST 2014
IPC: G01N 1/28
G02B 21/32
G02B 21/34
G02B 21/36
Applicants: LEICA MICROSYSTEMS CMS GMBH
Inventors: SCHLAUDRAFF, Falk
HOFFMANN, Florian
Title: METHOD FOR LASER MICRODISSECTION AND LASER MICRODISSECTION SYSTEM
Abstract:
A method for laser microdissection is proposed, in which a laser microdissection system (100) comprising a microscope (10) is used, wherein at least one dissected specimen (511) is separated from at least one object (51) to be dissected by means of a laser beam (b), which is focussed on the object (51) to be dissected through a microscope objective (41) of the microscope (10) by means of an epi-illumination apparatus (76) of the microscope (10) and said dissected specimen is collected in a dissected specimen collection container (55), wherein at least one collection surface (551) of the dissected specimen collection container (55) is acquired as an image, at least in sections, while image data (550) is generated. The collection surface (551) of the dissected specimen collection container (55) is acquired as an image at at least two times and the image data (550) produced in the process are compared to one another. A laser microdissection system (100) configured to perform such a method is likewise subject matter of the invention.