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1. (WO2014184805) BIOSENSOR FOR DETECTION OF MYCOTHIOL REDOX POTENTIAL
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2014/184805    International Application No.:    PCT/IN2014/000316
Publication Date: 20.11.2014 International Filing Date: 09.05.2014
IPC:
C07K 19/00 (2006.01), C12N 15/00 (2006.01), C12Q 1/26 (2006.01), C12Q 1/00 (2006.01), G01N 33/50 (2006.01), G01N 33/68 (2006.01)
Applicants: INTERNATIONAL CENTRE FOR GENETIC ENGINEERING & BIOTECHNOLOGY [IN/IN]; Aruna Asaf Ali Marg New Delhi 110067 (IN)
Inventors: SINGH, Amit; (IN).
BHASKAR, Ashima; (IN)
Agent: MALHOTRA, Kshitij; B703 Crown Appartments Plot 18B, Sector 7 Dwarka New Delhi 110075 (IN)
Priority Data:
1412/DEL/2013 13.05.2013 IN
Title (EN) BIOSENSOR FOR DETECTION OF MYCOTHIOL REDOX POTENTIAL
(FR) BIOCAPTEUR POUR LA DÉTECTION DU POTENTIEL REDOX DU MYCOTHIOL
Abstract: front page image
(EN)The disclosure provides a fusion construct that includes an engineered green fluorescent protein having a fluorescence spectrum that is sensitive to redox status and a mycothiol-dependent oxidoreductase linked to the engineered green fluorescent protein. The mycothiol-dependent oxidoreductase is a mycoredoxin identified from Mycobacterium tuberculosis strain H37Rv and the fusion construct can be used as a biosensor for detecting a change in a redox status of mycothiol in a cell.
(FR)L'invention concerne une construction hybride qui comprend une protéine de fluorescence verte génétiquement modifiée ayant un spectre de fluorescence qui est sensible au statut redox et une oxydoréductase dépendante du mycothiol liée à la protéine de fluorescence verte génétiquement modifiée. L'oxydoréductase dépendante du mycothiol est une mycoredoxine identifiée à partir de la souche H37Rv de Mycobacterium tuberculosis, et la construction hybride peut être utilisée comme biocapteur pour détecter un changement de statut redox du mycothiol dans une cellule.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)