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1. (WO2014178670) METHOD FOR SCREENING SURFACE STRUCTURE SUITABLE FOR CULTURING STEM CELL USING CULTURE VESSEL INCLUDING NANO GRADIENT PATTERN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2014/178670    International Application No.:    PCT/KR2014/003898
Publication Date: 06.11.2014 International Filing Date: 30.04.2014
IPC:
C12M 1/16 (2006.01), C12N 5/0735 (2010.01), C12Q 1/00 (2006.01)
Applicants: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION [KR/KR]; Korea Univ. Anam-ro 145 Seongbuk-gu Seoul 136-701 (KR)
Inventors: BAE, Dae Kyeong; (KR).
MOON, Sung Hwan; (KR).
LEE, Kyu Back; (KR).
PARK, Bo Gi; (KR).
CHUNG, Hyung Min; (KR)
Agent: LEE, Cheo Young; 11F., Yeosam Bldg. Taehaeran-ro 123 Gangnam-gu Seoul 135-748 (KR)
Priority Data:
10-2013-0048663 30.04.2013 KR
Title (EN) METHOD FOR SCREENING SURFACE STRUCTURE SUITABLE FOR CULTURING STEM CELL USING CULTURE VESSEL INCLUDING NANO GRADIENT PATTERN
(FR) PROCÉDÉ DE CRIBLAGE DE LA STRUCTURE D'UNE SURFACE CONVENANT À LA CULTURE DE CELLULE SOUCHE UTILISANT UN RÉCIPIENT DE CULTURE COMPRENANT UN PROFIL À NANO GRADIENT
(KO) 나노구배패턴을 포함하는 배양용기를 이용한 줄기세포 배양에 적합한 표면구조의 스크리닝 방법
Abstract: front page image
(EN)The present invention relates to a method for screening a surface structure suitable for culturing a stem cell including the step of culturing a cell in a cell culture vessel including a gradient nanopillar array with a diameter or spacing constantly varying with a difference of less than 1 nm; a cell culture vessel used in the screening method including nanopillars with a constantly increasing diameter uniformly arranged with a constantly decreasing spacing; and a method for culturing a stem cell maintaining stemness without a sustentacular cell including the step of culturing a cell in a cell culture vessel including the nanopillars selected by the screening method. The cell culture vessel including nanopillars with a constantly increasing diameter uniformly arranged with a constantly decreasing spacing according to the present invention can provide various surface configurations in a single culture vessel, so that the single culturing performed under the same condition makes it possible to simultaneously monitor the effects of the various surface configurations affecting the cell characteristics, thereby being capable of selecting the optimum surface configuration suitable for culturing a cell. The method selected through the screening for culturing a human embryonic stem cell can provide a method for proliferating cells maintaining stemness without coating an expensive extracellular substrate or using a sustentacular cell.
(FR)La présente invention concerne un procédé de criblage d'une structure de surface convenant à la culture d'une cellule souche comportant l'étape de culture d'une cellule dans un récipient de culture de cellules comprenant une rangée de nanopiliers de gradient ayant un diamètre ou un espacement variant constamment d'une différence inférieure à 1 nm ; un récipient de culture de cellules utilisé dans le procédé de criblage comprenant des nanopiliers d'un diamètre augmentant constamment disposés de manière uniforme selon un espacement réduisant constamment ; et un procédé de culture d'une cellule souche conservant le caractère souche sans cellule sustentaculaire comportant l'étape de culture d'une cellule dans un récipient de culture de cellules comprenant les nanopiliers sélectionnés par le procédé de criblage. Le récipient de culture de cellule comprenant les nanopiliers ayant un diamètre augmentant constamment disposés de manière uniforme selon un espacement réduisant constamment selon la présente invention peuvent fournir des configurations variées de surface dans un récipient de culture unique, de sorte que la culture unique effectuée sous les mêmes conditions permet de surveiller de manière simultanée les effets des diverses configurations de surface affectant les caractéristiques des cellules, ce qui permet de sélectionner la configuration de surface optimale convenant à la culture d'une cellule. Le procédé sélectionné au moyen du criblage permettant la culture d'une cellule souche embryonnaire humaine peut fournir un procédé de prolifération des cellules conservant le caractère souche sans enduction d'un substrat extracellulaire coûteux ni utilisation d'une cellule sustentaculaire.
(KO)본 발명은 직경(Diameter) 또는 간격(Spacing)이 1 nm 미만의 차이를 가지고 일정하게 변화하는 구배 나노기둥 어레이를 포함하는 세포배양용기에 세포를 배양하는 단계를 포함하는 줄기세포 배양에 적합한 표면구조를 스크리닝하는 방법, 상기 스크리닝 방법에 사용되는 일정하게 증가하는 직경을 갖는 나노기둥이 일정하게 감소하는 간격으로 균일하게 배열된 나노기둥을 포함하는 세포배양용기 및 상기 스크리닝 방법에 의해 선별된 조건의 나노기둥을 포함하는 세포배양용기에 세포를 배양하는 단계를 포함하는 지지세포 없이 줄기세포능(stemness)을 유지하면서 줄기세포를 배양하는 방법에 관한 것이다. 본 발명의 일정하게 증가하는 직경(Diameter)을 갖는 나노기둥이 일정하게 감소하는 간격(Spacing)으로 균일하게 배열된 나노기둥을 포함하는 세포배양용기는 하나의 배양용기 내에서 다양한 표면 형태를 제공할 수 있으므로, 동일한 조건에서 수행되는 단일 배양에 의해 표면 형태의 변화가 세포특성에 미치는 영향을 동시에 모니터링할 수 있으며, 이를 이용하여 세포배양에 적합한 최적의 표면형태를 선별할 수 있다. 상기 스크리닝을 통해 선별된 인간 배아줄기세포를 배양하는 방법은 값비싼 세포외 기질을 코팅하거나 지지세포를 이용할 필요없이 줄기세포능을 유지하면서 증식시킬 수 있는 방법을 제공할 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)