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1. (WO2014173955) IMPROVED GENE TARGETING AND NUCLEIC ACID CARRIER MOLECULE, IN PARTICULAR FOR USE IN PLANTS

Pub. No.:    WO/2014/173955    International Application No.:    PCT/EP2014/058246
Publication Date: Fri Oct 31 00:59:59 CET 2014 International Filing Date: Thu Apr 24 01:59:59 CEST 2014
IPC: C12N 15/82
Applicants: RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITÄT BONN
Inventors: HOMMELSHEIM, Carl
FRANTZESKAKIS, Lamprinos
ÜLKER, Bekir
Title: IMPROVED GENE TARGETING AND NUCLEIC ACID CARRIER MOLECULE, IN PARTICULAR FOR USE IN PLANTS
Abstract:
The present invention relates to a nucleic acid carrier molecule, comprising the general formula M-S1-L-W-S2, wherein M is a first polypeptide specifically binding to a donor nucleic acid sequence to be transferred into an organelle of a cell, W is a second polypeptide specifically binding to a target nucleic acid sequence, wherein said target nucleic acid sequence is located in an organelle of a cell, L is missing or is linking group allowing M and W flexibility and semi- independence, and S1 and S2 independently of each other are missing or are a signal peptide sequence, and can be fused to M and W proteins either N-or C-terminally, wherein said donor nucleic acid sequence is brought into close proximity with said target nucleic acid sequence when both nucleic acid sequences are bound to said carrier molecule. The present invention furthermore relates to methods for recombinantly transforming a nucleic acid into an organelle in a cell, preferably a plant cell, employing said nucleic acid carrier molecule.