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1. WO2014172527 - SYSTEMS AND METHODS FOR MULTIPLEXED BIOMARKER QUANTITATION USING SINGLE CELL SEGMENTATION ON SEQUENTIALLY STAINED TISSUE

Publication Number WO/2014/172527
Publication Date 23.10.2014
International Application No. PCT/US2014/034471
International Filing Date 17.04.2014
IPC
G06T 7/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
7Image analysis
CPC
G06K 9/0014
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
00127Acquiring and recognising microscopic objects, e.g. biological cells and cellular parts
0014Pre-processing, e.g. image segmentation
G06T 2207/10064
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
2207Indexing scheme for image analysis or image enhancement
10Image acquisition modality
10064Fluorescence image
G06T 2207/30024
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
2207Indexing scheme for image analysis or image enhancement
30Subject of image; Context of image processing
30004Biomedical image processing
30024Cell structures in vitro; Tissue sections in vitro
G06T 7/0012
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
7Image analysis
0002Inspection of images, e.g. flaw detection
0012Biomedical image inspection
Applicants
  • GENERAL ELECTRIC COMPANY [US]/[US]
Inventors
  • SANTAMARIA-PANG, Alberto
  • SARACHAN, Brion Daryl
  • RITTSCHER, Jens
  • PADFIELD, Dirk Ryan
  • CAN, Ali
  • PANG, Zhengyu
  • BELLO, Musodiq O
  • GINTY, Fiona Mary
  • SEVINSKY, Christopher James
  • LI, Qing
  • ROTHNEY, Megan Pearl
Agents
  • CHISHOLM, Robert
Priority Data
13/865,03617.04.2013US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SYSTEMS AND METHODS FOR MULTIPLEXED BIOMARKER QUANTITATION USING SINGLE CELL SEGMENTATION ON SEQUENTIALLY STAINED TISSUE
(FR) SYSTÈMES ET PROCÉDÉS DE QUANTIFICATION DE BIOMARQUEURS MULTIPLEXÉS À L'AIDE D'UNE SEGMENTATION DE CELLULE UNIQUE SUR UN TISSU SÉQUENTIELLEMENT COLORÉ
Abstract
(EN)
Improved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii) quantifying molecular protein profiles of individual cells in terms of sub cellular compartments (nuclei, membrane, and cytoplasm). The systems and methods of the present disclosure advantageously perform tissue segmentation at the sub-cellular level to facilitate analyzing, grouping and quantifying protein expression profiles of tissue in tissue sections globally and/or locally. Performing local-global tissue analysis and protein quantification advantageously enables correlation of spatial and molecular configuration of cells with molecular information of different types of cancer.
(FR)
La présente invention concerne des systèmes et des procédés améliorés permettant l'analyse d'images numériques. Plus particulièrement, la présente invention concerne des systèmes et des procédés améliorés permettant l'analyse d'images numériques d'échantillons de tissu biologique. Des modes de réalisation donnés à titre d'exemple consistent à : i) segmenter ii) grouper et iii) quantifier des profils de protéines moléculaires de cellules individuelles en termes de compartiments sous-cellulaires (noyaux, membrane et cytoplasme). Les systèmes et les procédés selon la présente invention réalisent de manière avantageuse une segmentation des tissus au niveau sous-cellulaire pour faciliter l'analyse, le groupement et la quantification, de manière globale et/ou locale, de profils d'expression protéique de tissus dans des sections de tissu. La réalisation locale et/ou globale d'une analyse des tissus et d'une quantification des protéines permet de manière avantageuse d'établir une corrélation entre une configuration spatiale et moléculaire de cellules et des informations moléculaires de différents types de cancer.
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