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1. WO2014167112 - A MUTANT SIALIDASE HAVING TRANS-SIALIDASE ACTIVITY FOR USE IN PRODUCTION OF SIALYLATED GLYCANS

Publication Number WO/2014/167112
Publication Date 16.10.2014
International Application No. PCT/EP2014/057422
International Filing Date 11.04.2014
IPC
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
C07K 14/435 (2006.01)
C12N 9/24 (2006.01)
CPC
A23L 33/10
A23L 33/40
A23V 2002/00
A61K 31/7016
A61K 31/702
C12N 9/2402
Applicants
  • DANMARKS TEKNISKE UNIVERSITET [DK/DK]; Anker Engelundsvej 1 Bygning 101A, 2. sal DK-2800 Kgs. Lyngby, DK
Inventors
  • MIKKELSEN, Jørn Dalgaard; DK
  • JERS, Carsten; DK
  • MICHALAK, Malwina; DK
  • KEPP, Kasper Planeta; DK
  • LARSEN, Dorte Møller; DK
Agents
  • SIMPSON, Verena; Guardian IP Consulting I/S Diplomvej, Building 381 DK-2800 Kgs. Lyngby, DK
Priority Data
13163551.812.04.2013EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A MUTANT SIALIDASE HAVING TRANS-SIALIDASE ACTIVITY FOR USE IN PRODUCTION OF SIALYLATED GLYCANS
(FR) SIALIDASE MUTANTE AYANT UNE ACTIVITÉ DE TRANS-SIALIDASE DESTINÉE À ÊTRE UTILISÉE EN PRODUCTION DE GLYCANES SIALYLÉS
Abstract
(EN)
The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo- saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.
(FR)
L'invention porte sur une enzyme mutante ayant une activité de trans-sialidase (EC 3.2.1.18), caractérisée par un rapport trans-sialidase:sialidase accru par comparaison avec son enzyme sialidase parente. En outre l'enzyme peut être utilisée dans un procédé pour la trans-sialylation de monosaccharides et d'oligosaccharides, notamment de galacto-oligosaccharides (GOS), de fructo-oligosaccharides (FOS), de malto-oligosaccharides (MOS), d'isomalto-oligosaccarides (IMO), de lactulose, de mélibiose, de maltose, de glycosylsaccharose, de lactosaccharose et de fucose. Les monosaccharides et oligosaccharides trans-sialylés produits à l'aide de l'enzyme mutante sont utiles en préparation de préparation pour nourrisson, de supplément nutritif prébiotique et de complément alimentaire.
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