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1. WO2014159847 - NANOPLASMONIC IMAGING TECHNIQUE FOR THE SPATIO-TEMPORAL MAPPING OF SINGLE CELL SECRETIONS IN REAL TIME

Publication Number WO/2014/159847
Publication Date 02.10.2014
International Application No. PCT/US2014/025316
International Filing Date 13.03.2014
IPC
G01N 33/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 2021/6491
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6491Measuring fluorescence and transmission; Correcting inner filter effect
G01N 21/274
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
27using photo-electric detection
274Calibration, base line adjustment, drift correction
G01N 21/554
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
55Specular reflectivity
552Attenuated total reflection
553and using surface plasmons
554detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
G01N 21/59
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
59Transmissivity
G01N 21/6458
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6456Spatial resolved fluorescence measurements; Imaging
6458Fluorescence microscopy
G01N 21/648
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
648using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
Applicants
  • THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF THE NAVY [US]/[US] (AllExceptUS)
  • RAPHAEL, Marc, P. [US]/[US] (US)
  • CHRISTODOULIDES, Joseph, A. [US]/[US] (US)
  • BYERS, Jeff, M. [US]/[US] (US)
  • DELEHANTY, James, B. [US]/[US] (US)
Inventors
  • RAPHAEL, Marc, P.
  • CHRISTODOULIDES, Joseph, A.
  • BYERS, Jeff, M.
  • DELEHANTY, James, B.
Agents
  • RESSING, Amy, L.
Priority Data
61/778,65213.03.2013US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NANOPLASMONIC IMAGING TECHNIQUE FOR THE SPATIO-TEMPORAL MAPPING OF SINGLE CELL SECRETIONS IN REAL TIME
(FR) TECHNIQUE D'IMAGERIE NANOPLASMONIQUE POUR LA CARTOGRAPHIE SPATIO-TEMPORELLE DE SÉCRÉTIONS CELLULAIRES ISOLÉES EN TEMPS RÉEL
Abstract
(EN)
A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
(FR)
L'invention concerne un procédé sans marqueur pour la cartographie spatio-temporelle de sécrétions protéiques à partir des cellules individuelles en temps réel par l'utilisation d'une puce pour l'imagerie par résonance plasmonique de surface localisée (LSPR). La puce est une lamelle en verre compatible pour l'utilisation dans un microscope de type standard ayant au moins un réseau de nanostructures plasmoniques fonctionnalisées dessinées sur celle-ci. Après le placement d'une cellule sur la puce, les sécrétions provenant de la cellule sont cartographiées spatialement et temporellement à l'aide de l'imagerie LSPR. L'imagerie par lumière émise et/ou l'imagerie de fluorescence peuvent être réalisées simultanément avec l'imagerie LSPR.
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