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1. WO2014159707 - AN EX VIVO HUMAN MULTIPLE MYELOMA CANCER NICHE AND ITS USE AS A MODEL FOR MULTIPLE MYELOMA

Publication Number WO/2014/159707
Publication Date 02.10.2014
International Application No. PCT/US2014/024847
International Filing Date 12.03.2014
IPC
C07K 14/78 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin (CIG)
C12N 5/09 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09Tumour cells
C12N 5/095 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09Tumour cells
095Stem cells; Progenitor cells
C12Q 1/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
CPC
C12M 23/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
02Form or structure of the vessel
16Microfluidic devices; Capillary tubes
C12N 5/0694
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0693Tumour cells; Cancer cells
0694Cells of blood, e.g. leukemia cells, myeloma cells
G01N 2500/10
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2500Screening for compounds of potential therapeutic value
10involving cells
G01N 2510/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2510Detection of programmed cell death, i.e. apoptosis
G01N 2800/52
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
52Predicting or monitoring the response to treatment; Prognosis
G01N 2800/7028
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
70Mechanisms involved in disease identification
7023(Hyper)proliferation
7028Cancer
Applicants
  • HACKENSACK UNIVERSITY MEDICAL CENTER [US]/[US]
  • THE TRUSTEES OF THE STEVENS INSTITUTE OF TECHNOLOGY [US]/[US]
Inventors
  • LEE, Woo
  • ZHANG, Wenting
  • TOLIAS, Peter
  • WANG, Hongjun
  • ZILBERBERG, Jenny
  • SIEGEL, David, Samuel
Agents
  • LUBIT, Beverly, W.
Priority Data
13/827,17014.03.2013US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) AN EX VIVO HUMAN MULTIPLE MYELOMA CANCER NICHE AND ITS USE AS A MODEL FOR MULTIPLE MYELOMA
(FR) NICHE DE CANCER DE MYÉLOME MULTIPLE HUMAIN EX VIVO ET SON UTILISATION COMME MODÈLE POUR LE MYÉLOME MULTIPLE
Abstract
(EN)
The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three- dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b )human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.
(FR)
La présente invention concerne une niche de cancer de myélome multiple (MM) dynamique ex vivo contenue dans un dispositif microfluidique. La niche de cancer de MM dynamique comprend (a) une construction tissulaire tridimensionnelle contenant une niche de moelle osseuse (MO) ex vivo dynamique, qui contient un tissu de type osseux minéralisé contenant des ostéoblastes viables auto-organisés en couches cellulaires multiples cohésives et une matrice extracellulaire sécrétée par les ostéoblastes adhérents viables; et un microenvironnement dynamiquement perfusé par des nutriments et des molécules de gaz dissous; et (b) des cellules de myélome humain ensemencées à partir d'une composition d'échantillon biologique comprenant des cellules mononucléaires et les cellules de myélome multiple. Les cellules de myélome humain sont en contact avec les ostéoblastes de la niche de MO, et la viabilité des cellules de myélome humain est maintenue par la niche de cancer de MM.
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