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1. WO2014147869 - METHOD FOR PREPARING CULTURE MEDIUM FOR CULTURING PLANT TISSUE, METHOD FOR CULTURING PLANT TISSUE, STERILIZING AGENT, GERMICIDAL AGENT, AND CULTURE MEDIUM COMPOSITION FOR CULTURING PLANT TISSUE

Publication Number WO/2014/147869
Publication Date 25.09.2014
International Application No. PCT/JP2013/075495
International Filing Date 20.09.2013
IPC
C12N 5/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04Plant cells or tissues
A01H 4/00 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques
A01N 25/12 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
25Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
12Powders or granules
CPC
A01H 4/001
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques ; ; Tissue culture techniques therefor
001Culture apparatus for tissue culture; Culture media therefor
A01N 1/0215
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0205Chemical aspects
021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
0215Disinfecting agents, e.g. antimicrobials for preserving living parts
Applicants
  • 水田 洋一 MIZUTA, Youichi [JP]/[JP]
Inventors
  • 水田 洋一 MIZUTA, Youichi
Agents
  • 特許業務法人京都国際特許事務所 KYOTO INTERNATIONAL PATENT LAW OFFICE
Priority Data
2013-06106422.03.2013JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR PREPARING CULTURE MEDIUM FOR CULTURING PLANT TISSUE, METHOD FOR CULTURING PLANT TISSUE, STERILIZING AGENT, GERMICIDAL AGENT, AND CULTURE MEDIUM COMPOSITION FOR CULTURING PLANT TISSUE
(FR) PROCÉDÉ DE PRÉPARATION D'UN MILIEU DE CULTURE POUR LA CULTURE D'UN TISSU VÉGÉTAL, PROCÉDÉ DE CULTURE DU TISSU VÉGÉTAL, AGENT DE STÉRILISATION, AGENT GERMICIDE, ET COMPOSITION DE MILIEU DE CULTURE POUR LA CULTURE D'UN TISSU VÉGÉTAL
(JA) 植物組織の培養用培地の製造方法及び植物組織の培養方法並びに滅菌処理剤、殺菌処理剤、及び植物組織培養用培地組成物
Abstract
(EN)
A method for preparing a culture medium for culturing a plant tissue, characterized by comprising: a step for boiling a culture medium; a step for adding a first sterilizing agent, which comprises multiple kinds of powdery chemicals, to the culture medium in the course of the boiling; a step for adding a second sterilizing agent, which comprises a single kind of chemical, to the culture medium at the point of the completion of the boiling, and then dispensing the culture medium into culture containers; a step for cooling the dispensed culture medium; a step for moistening the cooled culture medium and the plant tissue with multiple kinds of liquid chemicals; and a step for putting the plant tissue into the culture medium, said steps being conducted in this order. Thus, the present invention can provide a method for preparing a culture medium for culturing a plant tissue, whereby a sterilization treatment and a germicidal treatment can be easily carried out and the plant tissue can be grown on the same level as the case of using an autoclave or a clean bench, and a method for embedding a plant tissue.
(FR)
Cette invention concerne un procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, caractérisé en ce qu'il comprend : une étape consistant à porter un milieu de culture à ébullition ; une étape consistant à ajouter un premier agent de stérilisation, qui comprend de multiples types de produits chimiques à l'état pulvérulent, au milieu de culture pendant l'ébullition ; une étape consistant à ajouter un second agent de stérilisation, qui comprend un seul type de produit chimique, au milieu de culture au point d'achèvement de l'ébullition, puis à distribuer le milieu de culture dans des récipients de culture ; une étape consistant à refroidir le milieu de culture distribué ; une étape consistant à humidifier le milieu de culture refroidi et le tissu végétal à l'aide de multiples types de produits chimiques liquides ; et une étape consistant à placer le tissu végétal dans le milieu de culture, lesdites étapes étant exécutées dans cet ordre. Par conséquent, la présente invention concerne un procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, qui permet la mise en œuvre facile d'un traitement de stérilisation et d'un traitement germicide et la croissance du tissu végétal au même niveau en cas d'utilisation d'un autoclave ou d'un banc stérile, et un procédé d'ancrage d'un tissu végétal.
(JA)
 本発明の植物組織の培養用培地の製造方法は、培地を煮沸する工程と、煮沸途中で該培地に複数種の粉末状の薬剤から成る第1滅菌処理剤を添加する工程と、煮沸終了時に該培地に1種類の薬剤から成る第2滅菌処理剤を添加し、該培地を培養容器に分注する工程と、分注した培地を冷却する工程と、冷却した培地と植物組織を複数種の液体状の薬剤に濡らす工程と、当該培地に植物組織を入れる工程とを順に行うことを特徴とする。これにより、簡便に滅菌処理や殺菌処理を行うことができ、しかも、オートクレーブやクリーンベンチを使った場合と同程度に植物組織を生育することができる植物組織の培養用培地の製造方法と植物組織の置床方法を提供することができる。
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