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1. WO2014126233 - METHOD FOR ASSAYING MICRORNA, CANCER THERAPEUTIC AGENT, AND MEDICINAL COMPOSITION CONTAINING SAME FOR CANCER THERAPY

Publication Number WO/2014/126233
Publication Date 21.08.2014
International Application No. PCT/JP2014/053594
International Filing Date 17.02.2014
Chapter 2 Demand Filed 29.09.2014
IPC
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
A61K 31/713 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
7088Compounds having three or more nucleosides or nucleotides
713Double-stranded nucleic acids or oligonucleotides
A61K 33/24 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
33Medicinal preparations containing inorganic active ingredients
24Heavy metals; Compounds thereof
A61K 45/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
45Medicinal preparations containing active ingredients not provided for in groups A61K31/-A61K41/132
A61K 48/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 35/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35Antineoplastic agents
CPC
A61K 31/7088
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
7088Compounds having three or more nucleosides or nucleotides
A61K 31/713
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
7088Compounds having three or more nucleosides or nucleotides
713Double-stranded nucleic acids or oligonucleotides
A61K 33/243
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
33Medicinal preparations containing inorganic active ingredients
24Heavy metals; Compounds thereof
243Platinum; Compounds thereof
A61K 45/06
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
45Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
06Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
A61K 48/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 1/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
1Drugs for disorders of the alimentary tract or the digestive system
Applicants
  • 国立大学法人東京医科歯科大学 NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY [JP]/[JP]
Inventors
  • 稲澤 譲治 INAZAWA, Johji
  • 井上 純 INOUE, Jun
  • 山本 信祐 YAMAMOTO, Shinsuke
  • 河野 辰幸 KAWANO, Tatsuyuki
  • 小崎 健一 KOZAKI, Ken-ichi
Agents
  • 志村 光春 SHIMURA Mitsuharu
Priority Data
2013-02739915.02.2013JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR ASSAYING MICRORNA, CANCER THERAPEUTIC AGENT, AND MEDICINAL COMPOSITION CONTAINING SAME FOR CANCER THERAPY
(FR) PROCÉDÉ POUR L'ANALYSE DE MICRO-ARN, AGENT THÉRAPEUTIQUE ANTICANCÉREUX, ET COMPOSITION MÉDICINALE LE CONTENANT POUR UNE THÉRAPIE ANTICANCÉREUSE
(JA) マイクロRNAの測定方法、並びに、がん治療剤及びこれを含有するがん治療のための医薬組成物
Abstract
(EN) The present invention addresses the problem of finding a microRNA that strongly relates to the stabilization of NRF2 in a tumor and providing a means of utilizing the same in diagnosing and treating cancer. As the results of a screening of 470 kinds of microRNAs in a microRNA library with the use of HeLa cells, 8 kinds of microRNAs, each showing a large decrease in the activity compared with a control, and 8 kinds of microRNAs, each showing a large increase in the activity, were specified. It was discovered that, by using the microRNAs thus specified, the activation of NRF2 in a living body, in particular a tumor cell, can be detected and thus the malignancy, etc. of a tumor can be distinguished. Also, it was discovered that a nucleic acid containing a microRNA sequence relating to a decrease in the ARE activity as described above is usable as a cancer therapeutic agent.
(FR) La présente invention aborde le problème de trouver un micro-ARN qui s'associe fortement à la stabilisation de NRF2 dans une tumeur et de fournir un moyen d'utilisation de celui-ci dans le diagnostic et le traitement du cancer. Résultant d'un criblage de 470 sortes de micro-ARN dans une banque de micro-ARN par l'utilisation de cellules HeLa, 8 sortes de micro-ARN, chacun présentant une grande diminution de l'activité en comparaison à un témoin, et 8 sortes de micro-ARN, chacun présentant une grande augmentation de l'activité, ont été spécifiés. Il a été découvert que, par l'utilisation des micro-ARN ainsi spécifiés, l'activation de NRF2 dans un corps vivant, en particulier une cellule tumorale, peut être détectée et par conséquent la malignité, etc., d'une tumeur peut être distinguée. Également, il a été découvert qu'un acide nucléique contenant une séquence de micro-ARN associée à une diminution de l'activité ARE telle que décrite ci-dessus peut être utilisé comme agent thérapeutique anticancéreux.
(JA) 腫瘍におけるNRF2の安定化に強く関係するマイクロRNAを見出し、それらをがんの診断と治療に活用する手段を提供することを課題とする発明である。マイクロRNAライブラリーの470種類のマイクロRNAについて、HeLa細胞を用いたスクリーニングを行い、各々のマイクロRNAの活性値がコントロールに比べて大きく減少した8種類のマイクロRNAと、大きく上昇した8種類のマイクロRNAを特定した。そして、特定されたこれらのマイクロRNAを用いることによって、生体、特に腫瘍細胞、におけるNRF2の活性化を検出することが可能であり、これにより、腫瘍の悪性度等を鑑別することができることを見出した。さらに、上記ARE活性値の減少に係わるマイクロRNA配列を含む核酸を、がん治療剤として用いることができることを見出した。
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