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1. WO2014118247 - METHOD OF PRODUCING NANOBIOCATALYSTS

Publication Number WO/2014/118247
Publication Date 07.08.2014
International Application No. PCT/EP2014/051739
International Filing Date 29.01.2014
IPC
C12N 9/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
B01D 15/00 2006.1
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
C08G 18/64 2006.1
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
18Polymeric products of isocyanates or isothiocyanates
06with compounds having active hydrogen
28characterised by the compounds used containing active hydrogen
40High-molecular-weight compounds
64Macromolecular compounds not provided for by groups C08G18/42-C08G18/63104
C12N 11/14 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
14Enzymes or microbial cells immobilised on or in an inorganic carrier
B82Y 5/00 2011.1
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE  OR TREATMENT OF NANOSTRUCTURES
5Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
B82Y 40/00 2011.1
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE  OR TREATMENT OF NANOSTRUCTURES
40Manufacture or treatment of nanostructures
CPC
B82Y 5/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
5Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
C12N 11/14
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
14Enzymes or microbial cells immobilised on or in an inorganic carrier
C12N 9/0061
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0055acting on diphenols and related substances as donors (1.10)
0057with oxygen as acceptor (1.10.3)
0061Laccase (1.10.3.2)
C12Y 110/03002
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
110Oxidoreductases acting on diphenols and related substances as donors (1.10)
03with an oxygen as acceptor (1.10.3)
03002Laccase (1.10.3.2)
Applicants
  • INOFEA GMBH [CH]/[CH]
Inventors
  • HOMMES, Gregor
  • CORVINI, Philippe
  • CUMBO, Alessandro
  • SHAHGALDIAN, Patrick
  • DUDAL, Yves
Agents
  • LATSCHA, Silvan
Priority Data
13153276.430.01.2013EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF PRODUCING NANOBIOCATALYSTS
(FR) PROCÉDÉ DE PRODUCTION DE NANOBIOCATALYSEURS
Abstract
(EN) A method of producing nanobiocatalysts comprises: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths the method according to the invention allows for providing a continuous process and production of the nanobiocatalysts. Thereby, the production can be optimized regarding time, water, chemical and energy consumption. Also, the method according to the invention allows for a nanobiocatalysts production in which problems related to thixotrophy can be minimized. These effects can drastically increase the efficiency of the process thereby making production of nanobiocatalysts at a comparably high rate possible. Furthermore, the method according to the invention also allows for saving resources such that efficiency can also be increased in this regard.
(FR) L'invention concerne un procédé de production de nanobiocatalyseurs, comprenant : la suspension d'une matière de support dans un milieu liquide; la fonctionnalisation d'une surface de la matière de support par mélange de la matière de support en suspension avec un agent de fonctionnalisation pendant une première durée prédéterminée à une première température prédéterminée, un premier bouillon en résultant; l'application d'une protéine directement dans le premier bouillon après la première durée prédéterminée; le mélange de la protéine avec le premier bouillon et l'incubation de la protéine pendant une seconde durée prédéterminée à une seconde température prédéterminée, un second bouillon en résultant; l'immobilisation de la protéine incubée sur la surface fonctionnalisée de la matière de support, un troisième bouillon en résultant; la filtration du troisième bouillon; et la récupération d'un rétentat de la filtration. En traitant directement les premier et second bouillons, le procédé selon l'invention permet de fournir un procédé en continu et une production des nanobiocatalyseurs. Par conséquent, la production peut être optimisée du point de vue de la durée, de l'eau, de la consommation chimique et énergétique. Egalement, le procédé selon l'invention permet une production de nanobiocatalyseurs dans laquelle des problèmes associés à la thixotropie peuvent être rendus minimaux. Ces effets peuvent augmenter de façon drastique l'efficacité du procédé, produisant ainsi des nanobiocatalyseurs à une vitesse possible similairement élevée. De plus, le procédé selon l'invention peut également permettre d'économiser les ressources, de telle sorte que l'efficacité peut également être augmentée de ce point de vue.
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